CpG motifs present in bacterial DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and interferon gamma
CpG motifs present in bacterial DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and interferon gamma
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DOI：
10.1073/pnas.93.7.2879
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发表时间：
1996-04-02
影响因子：
11.1
通讯作者：
Krieg, AM
中科院分区：
文献类型：
--
作者：
Klinman, DM;Yi, AK;Krieg, AM
细菌感染可刺激宿主产生快速的炎症反应。由未甲基化的CpG二核苷酸和两侧的两个5'嘌呤和两个3'嘧啶组成的6碱基DNA基序通过诱导多克隆B细胞活化而促进这种反应。这种刺激基序在细菌DNA中比在高等脊椎动物中多20倍。目前的工作表明，相同的基序在体内和体外诱导白细胞介素（IL）6，IL-12和干扰素γ（但不是IL-2，IL-3，IL-4，IL-5或IL-10）的快速和协调的分泌。刺激性CpG DNA基序诱导B、T和自然杀伤细胞比脂多糖更有效地分泌细胞因子。因此，细菌DNA的免疫识别可能有助于细胞因子以及天然炎症反应特征的抗体产生。
Bacterial infection stimulates the host to mount a rapid inflammatory response, A 6-base DNA motif consisting of an unmethylated CpG dinucleotide flanked by two 5' purines and two 3' pyrimidines was shown to contribute to this response by inducing polyclonal B-cell activation, This stimulatory motif is 20 times more common in the DNA of bacteria than higher vertebrates. The current work shows that the same motif induces the rapid and coordinated secretion of interleukin (IL) 6, IL-12, and interferon gamma (but not IL-2, IL-3, IL-4, IL-5, or IL-10) in vivo and in vitro. Stimulatory CpG DNA motifs induced B, T, and natural killer cells to secrete cytokine more effectively than did lipopolysaccharide. Thus, Immune recognition of bacterial DNA may contribute to the cytokine as well as the antibody production characteristic of an Innate inflammatory response.