Second-site suppressor mutations of inactivating substitutions at gly247 of the tetracycline efflux protein, Tet(B).
Second-site suppressor mutations of inactivating substitutions at gly247 of the tetracycline efflux protein, Tet(B).
复制标题
四环素外排蛋白 Tet(B) 的 gly247 处失活取代的第二位点抑制突变。
DOI：
10.1128/jb.182.22.6514-6516.2000
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发表时间：
2000
影响因子：
3.2
通讯作者：
Levy,SB
中科院分区：
文献类型：
--
作者：
Saraceni-Richards,CA;Levy,SB
四环素外排蛋白泰特（B）跨膜螺旋8（TM-8）中Gly 247的Asp或Asn取代消除了四环素耐药性。部分恢复抗性的第二位点抑制突变位于TM-5、TM-8、TM-10或TM-11或细胞质环8-9或环10-11中。这些结果表明TM-8和泰特（B）的这些其他区域之间的物理接近或功能关系。
An Asp or Asn substitution for Gly247 in transmembrane helix 8 (TM-8) of Tet(B), the tetracycline efflux protein, eliminated tetracycline resistance. Second site suppressor mutations which partially restored resistance were located in TM-5, -8, -10, or -11 or in cytoplasmic loop 8-9 or loop 10-11. These results indicate physical proximity or functional relationships between TM-8 and these other regions of Tet(B).