A single gene and a pseudogene for the cellular tumour antigen p53
A single gene and a pseudogene for the cellular tumour antigen p53
复制标题
细胞肿瘤抗原 p53 的单基因和假基因
作者：
R. Zakut;M. Oren;B. Bienz;V. Lavie;S. Hazum;D. Givol
细胞肿瘤抗原p53是一种在多种转化细胞中水平升高的蛋白质（综述见参考文献1）。在由广谱试剂转化的细胞2 -7以及胚胎癌细胞3，8和自发转化体9，10中观察到p53的过度产生。虽然最初描述在小鼠2，3，9，类似的p53样蛋白也观察到其他物种的细胞，包括那些来自几个人类肿瘤11。在非转化细胞中，蛋白质转化非常迅速12，13，其水平似乎与细胞增殖相关14，15。到目前为止，人们对这种蛋白质和相应基因的确切性质知之甚少。我们现在提供的证据表明，存在一个单一的功能基因的小鼠p53和加工的假基因。还提供了鼠p53的预测氨基酸序列。
The cellular tumour antigen p53 is a protein found in elevated levels in a great variety of transformed cells (reviewed in ref. 1). Overproduction of p53 was observed in cells transformed by a wide spectrum of agents2–7 as well as in embryonal carcinoma cells3,8 and in spontaneous transformants9,10. Although initially described in mice2,3,9, similar p53-like proteins were also observed in cells of other species, including those derived from several human tumours11. In non-transformed cells the protein turns over very rapidly12,13 and its levels appear to correlate with cell proliferation14,15. Thus far, very little has been known about the precise nature of the protein and of the corresponding genes. We now provide evidence for the existence of a single functional gene for murine p53 and a processed pseudogene. The predicted amino acid sequence of murine p53 is also presented.