Trypsin and aminopeptidase gene expression is affected by age and food composition in Anopheles gambiae
Trypsin and aminopeptidase gene expression is affected by age and food composition in Anopheles gambiae
复制标题
DOI：
10.1016/s0965-1748(96)00014-8
复制
发表时间：
1996-07-01
影响因子：
3.8
通讯作者：
JacobsLorena, M
中科院分区：
文献类型：
--
作者：
Lemos, FJA;Cornel, AJ;JacobsLorena, M
本文研究了年龄和食物组成对冈比亚按蚊肠道胰蛋白酶和氨肽酶基因表达的影响。在刚羽化的雌蚊肠道中未检测到胰蛋白酶mRNA，但在出生后第一天胰蛋白酶mRNA就积累到较高水平。相比之下，在新羽化的雌性中观察到低的但显著的胰蛋白酶活性。亚细胞分级分离实验表明，腹胀诱导酶分泌到管腔中。血液而不是无蛋白质的膳食诱导新的胰蛋白酶mRNA和酶的积累。与胰蛋白酶不同，在新羽化的和年长的糖喂养的蚊子中检测到大量的氨肽酶活性。氨肽酶的亚细胞定位不随食物摄入而明显改变，酶活性的早期增加与食物组成无关。版权所有（C）1996 Elsevier Science Ltd.
The effects of age and food composition on the expression of trypsin and aminopeptidase genes in the Anopheles gambiae gut were investigated, No trypsin mRNA was detected in the gut of newly eclosed females, but this mRNA accumulated to relatively high levels within the first day of life. In contrast, low, but significant trypsin enzyme activity was observed in newly eclosed females, Subcellular fractionation experiments suggested that abdominal distention induces the secretion of the enzyme into the lumen, Blood, but not a protein-free meal, induced the accumulation of new trypsin mRNA and enzyme, Unlike trypsin, substantial aminopeptidase activity was detected in newly eclosed and in older, sugar fed mosquitoes, Moreover, the subcellular localization of aminopeptidase did not change appreciably with food ingestion, and the early increase of enzyme activity was independent of food composition. Copyright (C) 1996 Elsevier Science Ltd.