Evaluation of intraspecies genetic variation within the 60 kDa heat-shock protein gene (groEL) of Bartonella species
Evaluation of intraspecies genetic variation within the 60 kDa heat-shock protein gene (groEL) of Bartonella species
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DOI：
10.1099/00207713-49-3-1015
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发表时间：
1999-07-01
期刊：
影响因子：
--
通讯作者：
Regnery, RL
中科院分区：
文献类型：
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作者：
Marston, EL;Sumner, JW;Regnery, RL
基于 groEL 基因的 DNA 序列分析，对巴尔通体属的成员进行了系统发育研究，该基因编码 60 kDa 热休克蛋白 GroEL。确定了已建立的巴尔通体物种的 groEL 基因的近全长片段（1368 bp）的核苷酸序列数据，并用于推断种内系统发育关系。使用距离和简约方法从多个序列比对中推断出系统发育树，这证明了由六个得到良好支持的谱系组成的体系结构。结果与最近基于柠檬酸合酶（gltA）和先前观察到的基因型和表型特征的序列分析研究推导出的关系一致；然而，他们在属内水平上表现出了更大的统计支持。这表明 groEL 可能是巴尔通体谱系系统发育分析的更强大的工具。
A phylogenetic investigation was done on the members of the genus Bartonella, based on the DNA sequence analysis of the groEL gene, which encodes the 60 kDa heat-shock protein GroEL. Nucleotide sequence data were determined for a near full-length fragment (1368 bp) of the groEL gene of the established Bartonella species and used to infer intraspecies phylogenetic relationships. Phylogenetic trees were inferred from multiple sequence alignments by using both distance and parsimony methods, which demonstrated an architecture composed of six well-supported lineages. The results are consistent with relationships deduced from recent sequence analysis studies based upon citrate synthase (gltA) sind previously observed genotypic and phenotypic characteristics; however, they showed greater statistical support at the intragenus level. This suggests that groEL may be a more robust tool for phylogenetic analysis of Bartonella lineages.