Oral Cancer Diagnosis by Mechanical Phenotyping
Oral Cancer Diagnosis by Mechanical Phenotyping
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DOI：
10.1158/0008-5472.can-08-4073
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发表时间：
2009-03-01
期刊：
影响因子：
11.2
通讯作者：
Guck, Jochen
中科院分区：
文献类型：
--
作者：
Remmerbach, Torsten W.;Wottawah, Falk;Guck, Jochen
口腔鳞状细胞癌是10种最常见的癌症之一，5年后的致死率为50%。尽管很容易进入口腔进行癌症筛查，但成功治疗的主要限制是（前）恶性病变的预后标准不确定。鉴定功能性细胞标志物可能代表诊断和治疗的显著改善。为了实现这一目标，单个细胞的机械表型是一种新的方法来检测细胞骨架的变化，这是恶性变化的诊断。使用微流体光学拉伸器测量来自健康供体和癌症患者的细胞系和原始样品的细胞的顺应性。癌细胞表现出显著不同的机械行为，具有更高的平均变形性和增加的方差。癌细胞（从每个患者中测量n约30个细胞）的顺应性平均比健康供体的顺应性高3.5倍[D-正常=（4.43 +/- 0.68）10（-3）Pa-1; D-癌症=（15.8 +/- 1.5）10（-3）Pa-1; p < 0.01]。通过标准组织病理学证实患者样本的诊断结果。两个正常和四个癌症口腔上皮细胞系的测量支持这些发现的一般性。我们的研究结果表明，机械表型是一种明智的，无标记的方法分类癌细胞，使广泛的筛选可疑病变的口腔。原则上，它可以应用于任何癌症，以帮助常规诊断程序。[Cancer Res 2009;69（5）：1728-32]
Oral squamous cell carcinomas are among the 10 most common cancers and have a 50% lethality rate after 5 years. Despite easy access to the oral cavity for cancer screening, the main limitations to successful treatment are uncertain prognostic criteria for (pre-)malignant lesions. Identifying a functional cellular marker may represent a significant improvement for diagnosis and treatment. Toward this goal, mechanical phenotyping of individual cells is a novel approach to detect cytoskeletal changes, which are diagnostic for malignant change. The compliance of cells from cell lines and primary samples of healthy donors and cancer patients was measured using a microfluidic optical stretcher. Cancer cells showed significantly different mechanical behavior, with a higher mean deformability and increased variance. Cancer cells (n approximate to 30 cells measured from each patient) were on average 3.5 times more compliant than those of healthy donors [D-normal = (4.43 +/- 0.68) 10(-3) Pa-1; D-cancer = (15.8 +/- 1.5) 10(-3) Pa-1; p < 0.01]. The diagnosis results of the patient samples were confirmed by standard histopathology. The generality of these findings was supported by measurements of two normal and four cancer oral epithelial cell lines. Our results indicate that mechanical phenotyping is a sensible, label-free approach for classifying cancer cells to enable broad screening of suspicious lesions in the oral cavity. It could in principle be applied to any cancer to aid conventional diagnostic procedures. [Cancer Res 2009;69(5):1728-32]