DNA-alginate complex recognized by autoantibodies against DNA.
DNA-alginate complex recognized by autoantibodies against DNA.
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DNA-藻酸盐复合物可被 DNA 自身抗体识别。
DOI：
10.1016/s0141-8130(97)01146-x
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发表时间：
1997
影响因子：
8.2
通讯作者：
N. Nishi
中科院分区：
文献类型：
--
作者：
H. Kitamura;E. Matsuura;A. Nagata;N. Sakairi;S. Tokura;N. Nishi
双链DNA与海藻酸有效地复合并固定在聚苯乙烯微量滴定板表面。在酶联免疫吸附试验（ELISA）中观察到抗DNA自身抗体与固相DNA-藻酸盐复合物的剂量依赖性结合。与此相反，抗体与藻酸盐的非特异性结合几乎没有检测到比多聚赖氨酸。这些结果表明固相DNA-藻酸盐复合物在ELISA中作为抗原用于检测抗DNA抗体的可用性。
Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected ratheer than to poly-l-lysine. These results show an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.