NEUROPEPTIDES MODULATE COMPOUND POSTSYNAPTIC POTENTIALS IN BASOLATERAL AMYGDALA
NEUROPEPTIDES MODULATE COMPOUND POSTSYNAPTIC POTENTIALS IN BASOLATERAL AMYGDALA
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DOI：
10.1016/j.neuroscience.2009.09.061
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发表时间：
2009-12-29
期刊：
影响因子：
3.3
通讯作者：
Moore, S. D.
中科院分区：
文献类型：
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作者：
Chung, L.;Moore, S. D.
先前的行为研究表明，杏仁核固有的神经肽可以在恐惧和焦虑状态后发挥作用。我们之前已经表明，焦虑神经肽胆囊收缩素（CCK）增加了杏仁核基底外侧的抑制性神经传递。我们已经观察到CCK诱导由复合突触后电位（cPSPs）组成的同步节律活动。我们现在通过在5 mM细胞外K+中常规诱导cPSPs进一步表征了这些cPSPs。CCK在幼龄和成年大鼠脑切片中均以剂量依赖性的方式促进cPSP的发生。谷氨酸受体拮抗剂（NBQX或del - ap5）或低浓度的GABA(A)受体拮抗剂（bicuculline methiodide (BMI)， SR95531或picrotoxin）可减弱cPSPs，但GABA(B)受体拮抗剂CGP52432不能减弱cPSPs。低浓度的河豚毒素（TTX, 10 nM）也能减弱cpsp。Na-K-2Cl共转运体阻断剂布美他尼（11或10 μ M）也能阻断cpsp。促焦虑神经肽促肾上腺皮质激素释放因子（CRF）促进cPSPs，而抗焦虑神经肽（神经肽Y （NPY）和生长抑素）减弱cPSPs。苯二氮卓类激动剂地西泮剂量依赖性调节cpsp。甲氟喹在应用10分钟内促进cpsp。我们假设cpsp是由中间神经元子集和谷氨酸能投射神经元子集之间的正反馈产生的。由Elsevier Ltd代表IBRO出版。
Previous behavioral studies have shown that neuropeptides intrinsic to the amygdala formation can after fear and anxiety states. We have previously shown that the anxiogenic neuropeptide cholecystokinin (CCK) increases inhibitory neurotransmission in basolateral amygdala. We have since observed that CCK induces synchronized rhythmic activity composed of compound postsynaptic potentials (cPSPs). We have now further characterized these cPSPs by inducing cPSPs routinely in 5 mM extracellular K+. CCK facilitated cPSP occurrence in a dose dependent manner in brain slices from both young and mature rats. The cPSPs were attenuated by glutamate receptor antagonists (NBQX or DL-AP5) or low concentrations of GABA(A) receptor antagonists (bicuculline methiodide (BMI), SR95531, or picrotoxin), but not by the GABA(B) receptor antagonist, CGP52432. Low concentrations of tetrodotoxin (TTX, 10 nM) also attenuated the cPSPs. The Na-K-2Cl cotransporter blocker, bumetanide (11 or 10 mu M) also blocked the cPSPs. The anxiogenic neuropeptide corticotropin-releasing factor (CRF) facilitated cPSPs while anxiolytic neuropeptides (neuropeptide Y (NPY) and somatostatin) attenuated cPSPs. The benzodiazepine agonist diazepam dose-dependently modulated cPSPs. Mefloquine facilitated cPSPs within 10 min of application. We hypothesize that cPSPs are generated by positive feedback between a subset of interneurons and a subset of glutamatergic projection neurons. Published by Elsevier Ltd on behalf of IBRO.