Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori
Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori
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DOI：
10.1038/16495
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发表时间：
1999-01-14
期刊：
影响因子：
64.8
通讯作者：
Trost, TJ
中科院分区：
文献类型：
--
作者：
Alm, RA;Ling, LSL;Trost, TJ
幽门螺杆菌是人类最常见的细菌病原体之一，它在胃粘膜上定植，除非病人得到治疗，否则它似乎会在宿主的一生中持续存在。定植诱导慢性胃炎症，并可发展为多种疾病，严重程度从浅表性胃炎和消化性溃疡到胃癌和粘膜相关淋巴瘤(1)。菌株特异性遗传多样性已被提出参与生物体引起不同疾病的能力，甚至对被感染的宿主有益（2,3），并参与感染的终身慢性(4)。在这里，我们比较了两个不相关的幽门螺杆菌分离株的完整基因组序列。据我们所知，这是第一次这样的基因组比较。人们认为幽门螺杆菌具有很大程度的基因组和等位基因多样性，但我们发现这两种菌株的总体基因组组织、基因顺序和预测蛋白质组（基因组编码的蛋白质组）非常相似，每种菌株特异性基因的比例在6 - 7%之间，其中几乎一半的基因聚集在一个高变区。
Helicobacter pylori, one of the most common bacterial pathogens of humans, colonizes the gastric mucosa, where it appears to persist throughout the host's life unless the patient is treated. Colonization induces chronic gastric inflammation which can progress to a variety of diseases, ranging in severity from superficial gastritis and peptic ulcer to gastric cancer and mucosal-associated lymphoma(1). Strain-specific genetic diversity has been proposed to be involved in the organism's ability to cause different diseases or even be beneficial to the infected host(2,3) and to participate in the lifelong chronicity of infection(4). Here we compare the complete genomic sequences of two unrelated H. pylori isolates. This is, to our knowledge, the first such genomic comparison. H. pylori was believed to exhibit a large degree of genomic and allelic diversity, but we find that the overall genomic organization, gene order and predicted proteomes (sets of proteins encoded by the genomes) of the two strains are quite similar, Between 6 to 7% of the genes are specific to each strain, with almost half of these genes being clustered in a single hypervariable region.