INTRACELLULAR CAMPYLOBACTER-LIKE ORGANISM FROM FERRETS AND HAMSTERS WITH PROLIFERATIVE BOWEL-DISEASE IS A DESULFOVIBRIO SP
INTRACELLULAR CAMPYLOBACTER-LIKE ORGANISM FROM FERRETS AND HAMSTERS WITH PROLIFERATIVE BOWEL-DISEASE IS A DESULFOVIBRIO SP
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DOI：
10.1128/jcm.32.5.1229-1237.1994
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发表时间：
1994-05-01
影响因子：
9.4
通讯作者：
MURPHY, JC
中科院分区：
文献类型：
--
作者：
FOX, JG;DEWHIRST, FE;MURPHY, JC
增殖性肠病是一种与细胞内弯曲杆菌样生物(ICLO)的存在有关的多种家畜的肠道疾病。我们通过16S rRNA序列分析对从一只患有增殖性结肠炎的雪貂中提取的ICLO进行了鉴定。在这只雪貂中，含有ICLO的增生性肠道组织已经转移到肠系膜淋巴结、大网膜和肝脏。用通用原核引物从感染的肠外组织中扩增出ICLO的16S rRNA基因。用循环测序法对扩增出的16S rRNA基因的约1480个碱基进行了测序。与我们数据库中400多个细菌的序列比较表明，ICLO的序列与脱硫弧菌的序列最接近(87.5%的相似性)。对来自相关属的12个种和20个种的系统发育分析表明，ICLO与脱硫弧菌和其他5个种同属一个亚群。我们将这种生物称为胞内脱硫弧菌(IDO)。为扩增增生性肠道组织中IDO基因16S rRNA基因550个碱基的片段，设计了特异性引物。这一独特的550碱基片段是从9只患有ICLO相关疾病的雪貂和3只患ICLO相关疾病的仓鼠的冰冻肠道组织样本中扩增出来的，但不是从4个没有ICLO相关疾病的动物的肠道组织样本中扩增出来的。从一只仓鼠和一只雪貂的肠道组织中扩增出550个碱基的扩增产物，并进行了全序列测定。雪貂的IDO部分序列与先前测定的16S rRNA序列在长度上相同，而仓鼠的IDO序列相差一个碱基。在猪的增生性肠道组织中也发现了相同的细胞内微生物，并在组织培养中成功地保持了这种微生物。对这种胞内脱硫弧菌进行基于聚合酶链式反应(PCR)检测的特异引物的可获得性，将有助于确定其在多种感染宿主的增殖性肠病发病机制中的作用。
Proliferative bowel disease is an intestinal disorder of a variety of domestic animals associated with the presence of an intracellular Campylobacter-like organism (ICLO). We have identified the ICLO obtained from a ferret with proliferative colitis by 16S rRNA sequence analysis. In this ferret, proliferative bowel tissue containing the ICLO had translocated to the mesenteric lymph nodes, omentum, and liver. The 16S rRNA genes of the ICLO were amplified from an infected fragment of extraintestinal tissue by using universal prokaryotic primers. Approximately 1,480 bases of the amplified 16S rRNA gene were sequenced by cycle sequencing. Comparison of the sequence of the ICLO with those of over 400 bacteria in our data base indicated that the sequence of the ICLO was most closely related to that of Desulfovibrio desulfuricans (87.5% similarity). Phylogenetic analysis with 12 Desulfovibrio species and 20 species from related genera placed the ICLO in a subcluster within the genus Desulfovibrio with D. desulfuricans and 5 other Desulfovibrio species. We will refer to this organism as the intracellular Desulfovibrio organism (IDO). Specific primers were produced for PCR amplification of a 550-base Fragment of the 16S rRNA gene of the IDO in proliferative intestinal tissue samples. This unique 550-base segment was amplified from samples of frozen intestinal tissue from nine ferrets and three hamsters with ICLO-associated disease but not in four intestinal tissue samples from animals without the ICLO-associated disease. The 550-base amplified products from the bowel tissues of one hamster and one ferret were fully sequenced. The ferret IDO partial sequence was identical to the previously determined 16S rRNA sequence over its length, and the hamster IDO sequence differed by a single base. The same intracellular organism has been identified in proliferative intestinal tissues of swine and that the organism has been successfully maintained in tissue culture. The availability of specific primers for PCR-based detection of this intracellular Desulfovibrio organism will aid in the determination of its role in the pathogenesis of proliferative bowel disease in a variety of infected hosts.