Discovery and mapping of wheat Ph1 suppressors
Discovery and mapping of wheat Ph1 suppressors
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DOI：
10.1534/genetics.106.058115
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发表时间：
2006-09-01
期刊：
影响因子：
3.3
通讯作者：
Luo, M. -C.
中科院分区：
文献类型：
--
作者：
Dvorak, J.;Deal, K. R.;Luo, M. -C.
小麦（Triticum turgidum和T. aestivum）同源染色体间的配对是由5B染色体长臂上Ph1位点的表达所阻止的。稻瘟病菌基因组抑制小麦基因中Phl的表达。speltoides混合动力车。具有主要作用的抑制基因被定位为伊蚊长臂上的孟德尔位点。球茎染色体3S和7S。其中染色体3S位点命名为Su1-Ph1，染色体7S位点命名为Su2-Ph1。在5S染色体短臂上定位了一个影响较小的QTL，命名为QPh.ucd-5S。Su1-Ph1和Su2-Ph1的表达增加了冬稻的同源染色体配对。球茎杂交种的杂交优势分别为8.4和5.8个交点/细胞。Su1-Ph1对su2 - ph1是完全上位性的，这两个基因的共同作用增加了aestivum X Ae的同源染色体配对。与单独作用的Su1-Ph1水平相同。QPh。ucd-5S的表达增加了T. aestivum X Ae同源染色体配对1.6个交叉/细胞。并可加性地表达Su2-Ph1。假设Su1-Phl和Su2-Ph1的产物通过调节Ph1的表达而不是QPh的产物来影响同源染色体之间的配对。ucd-5S可能主要调控同源染色体之间的重组。
Pairing between wheat (Triticum turgidum and T. aestivum) homeologous chromosomes is prevented by the expression of the Ph1 locus on the long arm of chromosome 5B. The genome of Aegilops speltoides suppresses Phl expression in wheat X Ae. speltoides hybrids. Suppressors with major effects were mapped as Mendelian loci on the long arms of Ae. speltoides chromosomes 3S and 7S. The chromosome 3S locus was designated Su1-Ph1 and the chromosome 7S locus was designated Su2-Ph1. A QTL with a minor effect was mapped on the short arm of chromosome 5S and was designated QPh.ucd-5S. The expression of Su1-Ph1 and Su2-Ph1 increased homeologous chromosome pairing in T. aestivurn X Ae. speltoides hybrids by 8.4 and 5.8 chiasmata/cell, respectively. Su1-Ph1 was completely epistatic to Su2-Phl, and the two genes acting together increased homeologous chromosome pairing in T. aestivum X Ae. speltoides hybrids to the same level as Su1-Ph1 acting alone. QPh.ucd-5S expression increased homeologous chromosome pairing by 1.6 chiasmata/cell in T. aestivum X Ae. speltoides hybrids and was additive to the expression of Su2-Ph1. It is hypothesized that the products of Su1-Phl and Su2-Ph1 affect pairing between homeologous chromosomes by regulating the expression of Ph1 but the product of QPh.ucd-5S may primarily regulate recombination between homologous chromosomes.