Spatial segregation of degradation- and recycling-trafficking pathways in COS-1 cells
Spatial segregation of degradation- and recycling-trafficking pathways in COS-1 cells
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DOI：
10.1016/j.bbrc.2007.06.101
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发表时间：
2007-08-31
影响因子：
3.1
通讯作者：
Taguchi, Tomohiko
中科院分区：
文献类型：
--
作者：
Misaki, Ryo;Nakagawa, Takatoshi;Taguchi, Tomohiko
内吞作用后，大多数膜蛋白和脂质返回质膜（循环途径），但一些膜成分被传递给溶酶体（降解途径）。这两种途径在早期核内体中分化。回收途径包括回收核内体，降解途径包括晚期核内体和溶酶体。在许多细胞系中，这些细胞器通常位于核周区域，在那里它们在视觉上混合在一起。本研究通过追踪特异性配体（表皮生长因子和转铁蛋白）和Rab蛋白（Rab5、Rab7和Rab 11）的表达，证明在COS- 1细胞中，这两种途径在空间上是分离的。循环核内体大多局限于高尔基复合体的环状结构（“高尔基环”）内，而晚期核内体和溶酶体则被排除在高尔基环内。因此，COS- 1细胞内吞细胞器的独特组织结构可用于详细可视化内吞运输途径。(c) 2007爱思唯尔公司版权所有。
After endocytosis, most membrane proteins and lipids return to the plasma membrane (recycling pathway), but some membrane components are delivered to lysosomes (degradation pathway). These two pathways diverge in early endosomes. The recycling pathway involves recycling endosomes and the degradation pathway incorporates late endosomes and lysosomes. In many cell lines, these organelles often are located in the perinuclear region where they visually intermix. The present study, by tracking specific ligands (epidermal growth factor and transferrin) and expression of Rab proteins (Rab5, Rab7, and Rab 11), demonstrated that, in COS- 1 cells, the two pathways were spatially segregated. Recycling endosomes were mostly confined within the ring-shaped structure of the Golgi complex ("the Golgi ring"), whereas late endosomes and lysosomes were excluded from inside the Golgi ring. Thus, the unique organization of endocytic organelles in COS- 1 cells can be utilized to visualize endocytic trafficking pathways in detail. (c) 2007 Elsevier Inc. All rights reserved.