PROTEIN DETERMINANTS OF MYELINATION IN DIFFERENT REGIONS OF DEVELOPING RAT CENTRAL NERVOUS-SYSTEM
PROTEIN DETERMINANTS OF MYELINATION IN DIFFERENT REGIONS OF DEVELOPING RAT CENTRAL NERVOUS-SYSTEM
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DOI：
10.1042/bj1620247
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发表时间：
1977-01-01
影响因子：
4.1
通讯作者：
SMITH, ME
中科院分区：
文献类型：
--
作者：
BANIK, NL;SMITH, ME
几种不同的蛋白质决定因素与髓鞘形成的时间和速度在5个地区的中枢神经系统的测量。蛋白质沉积在亚细胞级分对应于成人髓鞘的密度，髓鞘的基本蛋白质特征的外观，个别髓鞘蛋白质的比例的变化，髓鞘标记2“：3”-环核苷酸3“-磷酸水解酶的外观和分布和纯化的髓鞘的形态[EM]研究的结果进行了比较。根据这些不同的标准，并与其他人的形态学观察相一致，髓鞘最早出现在脊髓中，然后出现在脑干中，最晚出现在大脑半球中。早在5日龄的大鼠脑干和脊髓中就观察到多层髓鞘。单个髓鞘蛋白的相对比例随着髓鞘成熟而变化，在所有区域中，较大的碱性蛋白随着较小的碱性蛋白的增加而减少。Wolfgram蛋白的比例也随着成熟而下降。较大比例的酶2 ''：3 ''-环核苷酸3 ''-磷酸水解酶在早期位于微粒体部分。在发展过程中的酶活性逐渐变得相关的一部分，对应于成年髓鞘的密度，这表明存在的前体膜片段的微粒体组分在髓鞘形成的早期阶段之前，紧凑的髓鞘形成。一个显着比例的总核苷酸磷酸水解酶活性的匀浆不能恢复在亚细胞级分在早期的年龄，但酶的回收率随着成熟和活性被发现更多的髓鞘馏分。
Measurements of several different protein determinants correlated with the time and rate of myelination in 5 areas of the CNS were presented. The deposition of protein in the subcellular fraction corresponding to the density of adult myelin, the appearance of basic protein characteristic of myelin, the change in proportions of the individual myelin proteins, the appearance and distribution of the myelin marker 2'':3''-cyclic nucleotide 3''-phosphohydrolase and the results of morphological [EM] studies of purified myelin were compared. According to these various criteria, and in agreement with the morphological observations of others, myelin appeared earliest in the spinal cord, then in the brain stem and latest in the cerebral hemispheres. Multilamellar myelin was observed in the rat brain stem and spinal cord as early as 5 days of age. The relative proportion of the individual myelin proteins changed with myelin maturation in all areas, with the larger basic protein decreasing reciprocally with increase of the smaller basic protein. The proportion of Wolfgram protein also decreased with maturation. Larger proportions of the enzyme 2'':3''-cyclic nucleotide 3''-phosphohydrolase were located in the microsomal fraction at early ages. During development the enzyme activity gradually became associated more with a fraction of a density corresponding to adult myelin, suggesting the presence of precursor membrane fragments in microsomal fractions in the early stages of myelination before compact myelin formation. A significant proportion of the total nucleotide phosphohydrolase activity of the homogenate could not be recovered in subcellular fraction at early ages, but the recoveries of the enzyme increased with maturation and the activity was found more in the myelin fraction.