OXIDATIVE STRESS 3 is a chromatin-associated factor involved in tolerance to heavy metals and oxidative stress
OXIDATIVE STRESS 3 is a chromatin-associated factor involved in tolerance to heavy metals and oxidative stress
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DOI：
10.1111/j.1365-313x.2008.03717.x
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发表时间：
2009-02-01
期刊：
影响因子：
7.2
通讯作者：
Ow, David W.
中科院分区：
文献类型：
--
作者：
Blanvillain, Robert;Kim, Jong Heon;Ow, David W.
将芥菜cDNA表达文库导入裂殖酵母裂殖酵母中，筛选耐镉转化子。表达氧化应激3（OXS 3）或OXS 3样cDNA的转化体表现出对一系列金属和氧化化学品的耐受性增强。OXS 3属于一个蛋白家族，该家族共有一个高度保守的结构域，对应于一个推定的N-乙酰转移酶或巯基转移酶催化位点。在这个保守结构域中的突变消除了拟南芥OXS 3增强S.粟酒裂殖酵母，表明在胁迫耐受性的推定催化结构域的作用。AtOXS 3的胁迫敏感突变体和过表达系的增强的耐受性支持OXS 3在胁迫耐受性中的作用。标记B的表达。juncea和A.拟南芥OXS 3蛋白在植物细胞中显示了与染色质离散部分的核小体相关的亚核斑点图案。OXS 3可能作为染色质重塑因子参与应激反应。
A cDNA expression library from Brassica juncea was introduced into the fission yeast Schizosaccharomyces pombe to select for transformants tolerant to cadmium. Transformants expressing OXIDATIVE STRESS 3 (OXS3) or OXS3-Like cDNA exhibited enhanced tolerance to a range of metals and oxidizing chemicals. OXS3 belongs to a family of proteins that share a highly conserved domain corresponding to a putative N-acetyltransferase or thioltransferase catalytic site. Mutations within this conserved domain abolished the ability of Arabidopsis thaliana OXS3 to enhance stress tolerance in S. pombe, indicating a role in stress tolerance for the presumptive catalytic domain. A stress-sensitive mutant of AtOXS3 and enhanced tolerance of overexpression lines support the role of OXS3 in stress tolerance. The expression of tagged B. juncea and A. thaliana OXS3 proteins in plant cells revealed a subnuclear speckling pattern related to the nucleosome in discrete parts of the chromatin. It is possible that OXS3 might act as a chromatin remodeling factor for the stress response.