Computer-generated three-dimensional reconstructions of serially sectioned mouse embryos
Computer-generated three-dimensional reconstructions of serially sectioned mouse embryos
复制标题
DOI：
10.1046/j.1469-7580.1998.19330323.x
复制
发表时间：
1998-10-01
影响因子：
2.4
通讯作者：
Baldock, RA
中科院分区：
文献类型：
--
作者：
Kaufman, MH;Brune, RM;Baldock, RA
我们与一组计算机科学家和解剖学家一起开发了基于计算机的方法，不仅联合收割机了扫描电子显微镜和传统组织学的优势，而且提供了组织识别的额外维度。后者是通过描绘或“绘画”对组织和结构进行适当标记来实现的。单独分割的解剖学定义的组织可以用特定颜色突出显示，并单独或与其他适当标记的组织和器官组合观察。组织可以以任何方向显示，无论是作为计算机生成的组织切片上的透明覆盖，还是作为没有组织背景的3D图像。该系统的另一个特点是，计算机图形技术与3D眼镜相结合，现在也允许观众看到立体分析下的对象。这个设施已被发现是特别有帮助的，在提请注意拓扑关系，以前没有容易注意到。由于小鼠现在是哺乳动物模型的选择在许多领域的发展研究，这是至关重要的是，一个基本水平的技能，可在研究界在解释连续切片的材料，例如，为迅速扩大举行，其中基因表达研究发挥了重要作用。同样重要的是，要了解在这些早期发育阶段所看到的各种器官系统的分化方面发生的动态变化。我们在这里强调的是额外的信息，这是可能获得的使用这种工具，在我们看来，不能很容易地从扫描电子显微镜分析或通过研究常规的系列组织切片的小鼠胚胎发育的类似阶段。该方法已被开发为一个大型项目的一部分，以准备一个涵盖从受精到出生的所有阶段的小鼠发育解剖数据库，以便将基因表达和细胞谱系数据准确地空间映射到正常小鼠发育的数字地图集上。在本文中，我们将展示如何这个数字解剖图集也代表了一个有价值的教学辅助和研究工具，在解剖学。
We have been involved with a group of computer scientists and anatomists in the development of computer-based methodologies that not only combine the advantages of scanning electron microscopy and conventional histology, but provide the additional dimension of tissue recognition. The latter is achieved by the appropriate labelling of tissues and structures by delineation or 'painting'. Individually segmented anatomically defined tissues can be highlighted in a particular colour and viewed either in isolation or in combination with other appropriately labelled tissues and organs. Tissues can be shown in any orientation either as a transparent overlay on computer-generated histological sections or as 3-D images without the histological background. An additional feature of the system is that computer graphics technology combined with 3-D glasses now also allows the viewer to see the object under analysis in stereo. This facility has been found to be particularly helpful in drawing attention to topological relationships that had not previously been readily noted. As the mouse is now the mammalian model of choice in many areas of developmental research, it is of critical importance that a basic level of skill is available in the research community in the interpretation of serially sectioned material, for example, for the rapidly expanding held in which gene expression studies play a significant role. It is equally important that there is an understanding of the dynamic changes that occur in relation to the differentiation of the various organ systems seen in these early stages of development. What we emphasise here is the additional information that it is possible to gain from the use of this tool which, in our view, could not readily have been gained from the analysis of scanning electron micrographs or by studying conventional serial histological sections of similar stages of mouse embryonic development. The methodology has been developed as part of a large project to prepare a database of mouse developmental anatomy covering all stages from fertilisation to birth in order to allow the accurate spatial mapping of gene expression and cell lineage data onto the digital Atlas of normal mouse development. In this paper we show how this digital anatomical Atlas also represents a valuable teaching aid and research tool in anatomy.