Factors involved in vivo and in vitro maturation of canine oocytes
Factors involved in vivo and in vitro maturation of canine oocytes
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DOI：
10.1016/j.theriogenology.2004.03.004
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发表时间：
2005-01-01
期刊：
影响因子：
2.8
通讯作者：
Macis, D
中科院分区：
文献类型：
--
作者：
Luvoni, GC;Chigioni, S;Macis, D
家犬可能是一个有价值的模式，研究和发展辅助生殖分类相关的濒危犬科动物。然而，与其他哺乳动物物种相比，该物种的卵母细胞体外成熟效率非常低，这限制了生殖生物技术的发展，例如体外胚胎生产，冷冻保存或核移植。在犬种中，雌性配子具有独特的特征：卵母细胞暴露于高浓度的孕酮，在卵泡环境中，它以网状状态排卵，并在输卵管中恢复和完成减数分裂。因此，狗卵母细胞体外成熟的最佳条件可能与其他哺乳动物模型不同，在其他哺乳动物模型中，卵泡在第一次减数分裂的中期II阶段排卵卵母细胞，其中雌激素是主导激素。体外培养系统需要基于体内条件，以创造与卵母细胞发育生理发生相似的微环境，但目前对犬卵母细胞成熟的调控机制还知之甚少，本文就影响犬卵母细胞体外成熟和体内成熟的因素进行了综述，并对今后的研究方向提出了建议。(c)2004年爱思唯尔公司All rights reserved.
The domestic dog could be a valuable model for studying and developing assisted reproduction in taxonomically related endangered Canids. However, the efficiency of in vitro oocyte maturation is very low in this species compared to that of other mammalian species and this limits the development of reproductive biotechnologies, such as in vitro embryo production, cryopreservation, or nucleus transfer.In canine species the female gamete has unique characteristics: the oocyte is exposed to high concentration of progesterone, in the follicular environment, it is ovulated in the dictyate state, and resumes and completes meiosis in the oviduct. Therefore, optimum conditions for in vitro maturation of dog oocytes may differ from other mammalian models in which follicles, where estrogens are the dominant hormones, ovulate oocytes at the Metaphase II stage of the first meiotic division.An in vitro culture system needs to be based on in vivo conditions in order to create a microenvironment similar to that in which oocyte development occurs physiologically, but little is known on mechanisms regulating oocyte maturation in the dog.This review analyzes the known factors involved in canine oocyte maturation in vivo and in vitro in order, to suggest on which aspects future investigations may be focused. (c) 2004 Elsevier Inc. All rights reserved.