A direct gaseous ethanol imaging system for analysis of alcohol metabolism from exhaled breath
A direct gaseous ethanol imaging system for analysis of alcohol metabolism from exhaled breath
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DOI：
10.1016/j.snb.2013.05.071
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发表时间：
2013-09
影响因子：
8.4
通讯作者：
T. Arakawa;Xin Wang;Takumi Kajiro;K. Miyajima;Shuhei Takeuchi;H. Kudo;K. Yano;K. Mitsubayashi
中科院分区：
文献类型：
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作者：
T. Arakawa;Xin Wang;Takumi Kajiro;K. Miyajima;Shuhei Takeuchi;H. Kudo;K. Yano;K. Mitsubayashi
研制了一种无创呼出气乙醇气体成像系统。该系统提供直接、快速、无痛的患者采样，并可用于诊断疾病。该系统提供了一个图像的乙醇浓度使用化学发光（CL）从酶固定化支持。该系统通过乙醇氧化酶和辣根过氧化物酶-鲁米诺-过氧化氢体系诱导鲁米诺反应来测量乙醇浓度作为CL强度。通过电子倍增器EM-CCD相机检测和分析呼出气中乙醇产生的CL的时空变化。乙醇皮肤斑贴试验是一种简单的方法，用于指示ALDH 2（ALDH 2），在健康志愿者中进行。分析来自具有ALDH 2（+）和ALDH 2（-）的志愿者的呼吸样本。呼出气流量控制单元可以在没有气体采样袋的情况下控制和优化人类呼吸的自然不稳定的流量。在呼出气流速的变异系数估计为2.8%，流速为200 mL/min。从志愿者呼出的气态乙醇可视化和分析超过300分钟后，口服乙醇0.4 g/kg体重。对于ALDH 2（+）和ALDH 2（-）志愿者，呼出气乙醇浓度的峰值出现在30分钟时，然后呼气乙醇浓度在300分钟的剩余时间内逐渐降低。
A non-invasive breath gaseous ethanol imaging system with an exhaled breath flow rate control has been developed. The system offers straight-forward, rapid, and painless sampling from patients, and can be applied to diagnose diseases. The system provides an image of ethanol concentration using chemiluminescence (CL) from an enzyme-immobilized support. The system measures ethanol concentrations as intensities of CL by luminol reaction induced by alcohol oxidase and horseradish peroxidase–luminol–hydrogen peroxide system. The spatio-temporal change in CL generates by ethanol in an exhaled breath is detected by an electron multiplier EM-CCD camera and analyzed. An ethanol skin patch test, a simple method of indicating aldehydehydrogenase 2 (ALDH2), is performed on healthy volunteers. Breath samples from volunteers with ALDH2(+) and ALDH2(−) are analyzed. The exhaled breath flow rate control unit can control and optimize the naturally unstable flow rate of human breath without a gas sampling bag. The coefficient of variance in the exhaled breath flow rate is estimated at 2.8% at a flow rate of 200 mL/min. Exhaled gaseous ethanol obtained from volunteers is visualized and analyzed over a period of 300 min following the oral administration of ethanol at 0.4 g/kg body weight. A peak in the exhaled breath ethanol concentration appears at 30 min for both ALDH2(+) and ALDH2(−) volunteers, the breath ethanol concentration then gradually decreases over the remainder of the 300 min.