Abundance of Plant-Associated Gammaproteobacteria Correlates with Immunostimulatory Activity of Angelica sinensis.
Abundance of Plant-Associated Gammaproteobacteria Correlates with Immunostimulatory Activity of Angelica sinensis.
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DOI：
10.3390/medicines6020062
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发表时间：
2019-05-31
期刊：
影响因子：
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通讯作者：
Kawamura, Akira
中科院分区：
文献类型：
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作者：
Kalpana, Kriti;Montenegro, Diego;Kawamura, Akira
背景：当归是一种药用植物，具有多种生物学效应，包括刺激人体天然免疫细胞的能力。最近的研究表明，中华按蚊的免疫刺激活性来源于植物相关细菌的微生物相关分子模式(MAMPs)。然而，目前还不清楚中华按蚊中的哪些细菌分类群负责产生免疫刺激MAMPs。方法：对中华按蚊样品进行单核细胞刺激试验和16S核糖体RNA扩增序列测定，了解其免疫刺激活性和微生物群落。结果数据用线性判别分析效应大小(Lefse)进行分析，这是一个发现元基因组生物标记物的在线生物统计工具，以确定与免疫刺激活性相关的细菌分类特征。结果：革兰氏阳性菌、β蛋白杆菌、革兰氏阳性菌与免疫刺激活性呈负相关，革兰氏阳性菌与免疫刺激活性呈正相关。结论：已鉴定的细菌分类群为鉴定植物中具有免疫刺激作用的MAMPs开辟了新的阶段。
Background: Angelica sinensis is a medicinal plant known for a variety of biological effects, including its ability to stimulate innate immune cells in humans. Recent studies indicate that the immunostimulatory activity of A. sinensis arises from microbe-associated molecular patterns (MAMPs) of plant-associated bacteria. However, it is unknown which bacterial taxa in A. sinensis are responsible for the production of immunostimulatory MAMPs. Methods: Samples of A. sinensis were subjected to a cell-based assay to detect monocyte-stimulation and 16S ribosomal RNA amplicon sequencing, which revealed their immunostimulatory activity and microbial communities. The resulting data were analyzed by Linear discriminant analysis effect size (LEfSe), an online biostatistical tool for metagenomic biomarker discovery, to identify the bacterial taxonomical features correlated with the immunostimulatory activity. Results: A series of bacterial taxa under Gammaproteobacteria correlated positively with the immunostimulatory activity, whereas several Gram-positive taxa and Betaproteobacteria correlated negatively with the activity. Conclusions: The identified bacterial taxa set a new stage to characterize immunostimulatory MAMPs in plants.