Subcellular localization of DJ-1 in human HL-60 leukemia cells in response to diallyl disulfide treatment.
Subcellular localization of DJ-1 in human HL-60 leukemia cells in response to diallyl disulfide treatment.
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DOI：
10.3892/mmr.2016.5831
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发表时间：
2016-11
影响因子：
3.4
通讯作者：
Tan H
中科院分区：
文献类型：
--
作者：
Li Q;Tang Y;Qin J;Yi L;Yang Y;Wang J;He J;Su Q;Tan H
已证明二烯丙基二硫（DADS）在体外和体内具有强效的抗癌作用。研究表明DADS在体外可诱导人白血病细胞分化和/或凋亡。然而，这些抗癌作用的机制仍然难以捉摸。本研究旨在探讨DADS对人白血病HL-60细胞胞浆、胞核和线粒体内deglycase DJ-1（Parkinsonism associated deglycase-7，PARK-7）蛋白亚细胞定位的影响，为DADS抗白血病的分子机制提供新的实验依据。在不同时间点收集DADS诱导的HL-60细胞，并使用特异性细胞组分分离试剂盒从细胞质、细胞核和线粒体中分离蛋白。DJ-1的蛋白质表达水平在这些亚细胞级分的HL 60细胞暴露于DADS不同的时间长度后，确定使用蛋白质印迹，免疫细胞化学和免疫荧光技术。HL-60细胞暴露于1.25 mg/l DADS 8 h后，细胞质中DJ-1的蛋白表达水平显著降低，而细胞核组分中DJ-1的表达与未处理的对照组相比显著增加。5和10 mg/l DADS处理后，HL-60细胞线粒体DJ-1蛋白表达水平显著降低。这些结果表明，低浓度DADS可促进DJ-1蛋白从细胞质向细胞核转位，提示DJ-1可能在细胞分化过程中起转录因子或辅助因子结合蛋白的作用。DADS诱导HL-60细胞凋亡可能与线粒体DJ-1的表达有关。
Diallyl disulfide (DADS) has been demonstrated to exert potent anticancer effects in vitro and in vivo. Previous studies indicate that DADS may induce the differentiation and/or apoptosis of human leukemia cells in vitro. However, the mechanisms underlying these anticancer effects remain elusive. The aim of the present study was to investigate alterations in the subcellular localization of protein deglycase DJ-1 (also known as Parkinsonism associated deglycase-7, PARK-7) in the cytoplasm, nucleus and mitochondria of human leukemia HL-60 cells induced by DADS, in order to provide novel experimental evidence for the molecular mechanisms underlying the anticancer mechanisms of DADS in leukemia cells. HL-60 cells induced by DADS were collected at different time points, and proteins from the cytoplasm, nucleus and mitochondria of the cells were isolated using specific cellular component isolation kits. The protein expression levels of DJ-1 in these subcellular fractions of HL60 cells following exposure to DADS for varying lengths of time, were determined using western blotting, immunocytochemistry and immunofluorescence techniques. Following exposure of HL-60 cells to 1.25 mg/l DADS for 8 h, the protein expression levels of DJ-1 were significantly decreased in the cytoplasm, while nuclear fractions exhibited a significant increase in DJ-1 expression when compared with untreated controls. The protein expression levels of DJ-1 in mitochondria of HL-60 cells were significantly decreased following treatment with 5 and 10 mg/l DADS. These results demonstrate that exposure of HL-60 cells to low concentrations of DADS may promote DJ-1 protein translocation from the cytoplasm to the nucleus, which suggests that DJ-1 may function as a transcription factor or cofactor binding protein in the process of cell differentiation. The expression of DJ-1 in mitochondria may be associated with induction of apoptosis in HL-60 cells treated with moderate doses of DADS.
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DOI：
10.1158/1940-6207.capr-16-0044
发表时间：
2016-07
期刊：
Cancer prevention research (Philadelphia, Pa.)
影响因子：
--
作者：
Saud SM;Li W;Gray Z;Matter MS;Colburn NH;Young MR;Kim YS
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作者：
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10.1073/pnas.0607260103
发表时间：
2006-10-10
影响因子：
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作者：
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DOI：
10.1016/j.biocel.2011.09.007
发表时间：
2012-01-01
影响因子：
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作者：
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作者：
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