Abstract 119: Influence of Anesthetics on the Hemodynamic Response in a Rat Model of Hemorrhagic Shock
Abstract 119: Influence of Anesthetics on the Hemodynamic Response in a Rat Model of Hemorrhagic Shock
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摘要 119：麻醉药对失血性休克大鼠模型血流动力学反应的影响
DOI：
10.1161/circ.140.suppl_2.119
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发表时间：
2019
期刊：
影响因子：
37.8
通讯作者：
Riess Matthias L
中科院分区：
文献类型：
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作者：
Balzer C;Baudenbacher Franz J;Eagle Susan S;Salzman Michele M;Cleveland William J;Riess Matthias L
引言：大鼠失血性休克（HS）的实验模型对于测试可能改善人类结局的新疗法非常重要，在这些实验期间需要全身麻醉。已知挥发性麻醉剂异氟烷在大鼠HS模型中具有有益作用。关注心血管代偿机制，我们希望在轻度HS（2级）大鼠模型中评价异氟烷与注射麻醉剂戊巴比妥的比较。我们假设，异氟烷在HS的发展过程中，改善血流动力学相比，Pentobarbital.Methods：12 Sprague-Dawley大鼠最初麻醉与腹膜内（IP）注射戊巴比妥（45 mg/kg）和插管（1 L/min，FiO20.25）;心率（HR）通过皮下ECG针监测。股动脉和静脉分别插管用于连续血压测量和液体输送。在一组（n=7）中，通过重复IP注射戊巴比妥（剂量mg/kg）继续麻醉，另一组（n=5）接受连续异氟烷（1%）。稳定30分钟并给予肝素（100 IU/kg）后，通过股静脉在1分钟内取出1 ml血液诱导HS，每3分钟重复一次，直至取出5 ml血液。结果：在基线期，两组大鼠的心率和平均动脉压（MAP）均无显著性差异。5毫升的出血后，两组均表现出显着的变化相比，基线，具有显着较高的MAP和HR在大鼠只给Pentobarbital.Conclusions：在我们的大鼠模型HS，异氟烷抑制生理反应，以弥补轻度出血。异氟醚组大鼠的心血管反应是每毫升出血HR和MAP降低，而仅给予戊巴比妥的大鼠能够通过升高HR维持其MAP，直至失代偿。
Introduction:Experimental models of hemorrhagic shock (HS) in rats are important to test new treatments that may improve outcomes in humans, and general anesthesia is required during these experiments. The volatile anesthetic Isoflurane is known for its beneficial effects in rat HS models. Focusing on cardiovascular compensatory mechanisms, we wanted to evaluate Isoflurane versus the injectable anesthetic Pentobarbital in our rat model of mild HS (class 2). We hypothesize that Isoflurane during development of HS improves hemodynamics compared to Pentobarbital.Methods:Twelve Sprague-Dawley rats were initially anesthetized with an intraperitoneal (IP) injection of Pentobarbital (45 mg/kg) and intubated (1 L/min, FiO20.25); heart rate (HR) was monitored by subcutaneous ECG needles. Femoral artery and vein were cannulated for continuous blood pressure measurement and delivery of fluids, respectively. In one group (n=7), anesthesia was continued with repeated IP injections of Pentobarbital (dose mg/kg), the other group (n=5) received continuous Isoflurane (1%). After 30 min of stabilization and administration of Heparin (100 IU/kg), HS was induced by removal of 1 ml of blood over 1 min via the femoral vein, repeated every 3 min until a volume of 5 ml of blood was removed. Mean arterial blood pressure (MAP) and HR were recorded and analyzed in LabChart.Results:During baseline, rats showed no significant differences in HR and MAP between both groups. After 5 ml of hemorrhage, both groups showed significant changes compared to baseline, with significantly higher MAP and HR in rats given only Pentobarbital.Conclusions:In our rat model of HS, Isoflurane dampens the physiologic response to compensate for mild hemorrhage. The cardiovascular response of rats in the Isoflurane group was a decrease of HR and MAP to every ml of hemorrhage, while rats given only Pentobarbital were able to maintain their MAP by raising their HR until decompensation.