DNA-SEQUENCE AND TRANSCRIPTION OF A DNA MINICIRCLE ISOLATED FROM MALE-FERTILE SUGAR-BEET MITOCHONDRIA
DNA-SEQUENCE AND TRANSCRIPTION OF A DNA MINICIRCLE ISOLATED FROM MALE-FERTILE SUGAR-BEET MITOCHONDRIA
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DOI：
10.1093/nar/12.11.4747
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发表时间：
1984-01-01
影响因子：
14.9
通讯作者：
MARCKER, KA
中科院分区：
文献类型：
--
作者：
HANSEN, BM;MARCKER, KA
从雄性可育甜菜线粒体中分离出一个由 1440 bp 组成的小环。确定了小环的整个核苷酸序列。所有三个阅读框中都有几个终止密码子，因此小环不太可能编码多肽产物。然而，除了分别由1100和950个碱基组成的两个非常小的RNA产物之外，小环被转录产生由850个碱基组成的主要RNA产物。所有三种 RNA 产物均从同一条链转录。主要 RNA 产物的转录起始点通过 S1 核酸酶保护分析确定。
A minicircle consisting of 1440 bp was isolated from male fertile sugar beet mitochondria. The entire nucleotide sequence of the minicircle was determined. There are several stop codons in all three reading frames and it is therefore unlikely that the minicircle codes for a polypeptide product. However, the minicircle is transcribed yielding a major RNA product consisting of 850 bases in addition to two very minor RNA products of 1100 and 950 bases, respectively. All three RNA products are transcribed from the same strand. The starting point for transcription of the major RNA product was determined by S1 nuclease protection analysis.