Phenotypic Chemical Screening in CD4+ T Cells to Identify Epigenetic Inhibitors.
Phenotypic Chemical Screening in CD4+ T Cells to Identify Epigenetic Inhibitors.
复制标题
CD4 T 细胞表型化学筛选以鉴定表观遗传抑制剂。
DOI：
10.1007/978-1-0716-3397-7_17
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Cribbs AP
中科院分区：
文献类型：
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作者：
Cribbs AP
化学生物学提供了一种有吸引力的方法来识别参与特定生物过程的基因。这种筛选方法有其优点，因为测定通常是非破坏性的，即使作用机制未知，也可以进行分析。在免疫反应期间，细胞上调称为细胞因子的小蛋白质的表达和分泌，这些蛋白质对细胞之间的相互作用和通信具有特定影响。在这里，我们描述的原则和步骤，在执行化学筛选，以确定影响细胞因子的产生在分化的Th1，Th2和Th17细胞的表观遗传抑制剂。我们的方法为鉴定能够控制CD4+ T细胞细胞因子功能的表观遗传化学化合物提供了理论基础，这可能有利于治疗炎症性疾病。
Chemical biology provides an attractive approach to identify genes involved in a particular biological process. This screening approach has its advantages because the assays are usually non-destructive, and analysis can be performed even if the mechanism of action is unknown. During an immune reaction, cells upregulate the expression and secretion of small proteins called cytokines that have specific effects on the interactions and communication between cells. Here, we describe the principles and steps involved in the execution of chemical screening for identifying epigenetic inhibitors that affect cytokine production in differentiated Th1, Th2, and Th17 cells. Our approach provides a rationale for identifying epigenetic chemical compounds that are capable of controlling CD4+T-cell cytokine function that may be beneficial for treating inflammatory diseases.