In vitro gene delivery by degraded polyamidoamine dendrimers
In vitro gene delivery by degraded polyamidoamine dendrimers
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DOI：
10.1021/bc9600630
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发表时间：
1996-11-01
影响因子：
4.7
通讯作者：
Szoka, FC
中科院分区：
文献类型：
--
作者：
Tang, MX;Redemann, CT;Szoka, FC
已有报道使用DNA和球形阳离子聚酰胺胺聚合物(StarBurst树枝状大分子)之间的络合物来转染培养的细胞，这些聚合物由表面的伯胺和内部的叔胺组成。树枝状大分子在各种溶媒(如水或丁醇)中的热处理显著提高了(>；50倍)转染率。这种处理导致酰胺键上的树枝状大分子显著降解，导致分子量从很低(
Transfection of cultured cells has been reported using complexes between DNA and spherical cationic polyamidoamine polymers (Starburst dendrimers) that consist of primary amines on the surface and tertiary amines in the interior. The transfection activity of the dendrimers is dramatically enhanced (> 50-fold) by heat treatment in a variety of solvolytic solvents, e.g., water or butanol. Such treatment induces significant degradation of the dendrimer at the amide linkage, resulting in a heterodisperse population of compounds with molecular weights ranging from the very low (