Improvement of spatial resolution of elemental imaging using laser ablation-ICP-mass spectrometry
Improvement of spatial resolution of elemental imaging using laser ablation-ICP-mass spectrometry
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DOI：
10.1007/s44211-022-00085-8
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发表时间：
2022-03
影响因子：
1.6
通讯作者：
Eisei Tanaka;Takehisa Matsukawa;Yasuo Kuroki;Minoru Suzuki;K. Yokoyama;T. Hirata
中科院分区：
文献类型：
--
作者：
Eisei Tanaka;Takehisa Matsukawa;Yasuo Kuroki;Minoru Suzuki;K. Yokoyama;T. Hirata
激光烧蚀-ICP-质谱仪 (LA-ICPMS) 现在已成为对岩石、矿物、功能材料或生物组织样品中的主量元素到痕量元素进行图谱分析的最主要的分析技术之一。在本研究中，通过结合小体积细胞和偏移激光烧蚀方案进行成像分析，以提高空间分辨率。新设计的小体积池和炬内气体混合协议的结合提供了更快的信号冲洗时间（约 0.8 秒，将 238U 还原为百分之一，1% 的水平）。这对于提高激光扫描方向的空间分辨率非常重要。此外，激光线扫描之间的小距离（激光间距距离）与仅放置在玻璃基板上的生物组织样本的优先和完全烧蚀的组合导致激光烧蚀比激光烧蚀凹坑的尺寸更小的区域（剃须烧蚀）。通过剃须烧蚀，即使使用直径为 8 µm 的激光束，也可以实现更窄带宽（例如 2 µm）的激光线扫描。为了证明本技术的实际用途，对掺杂 Gd-乙二胺四亚甲基膦酸的小鼠骨进行了成像分析。本技术更清楚地识别了 Gd 在磷灰石细胞边缘的优先分布。较短的冲洗系统设置和剃须消融协议的结合使我们能够提高通过 LA-ICPMS 技术获得的元素成像的空间分辨率。图形摘要
Laser ablation-ICP-mass spectrometer (LA-ICPMS) now becomes one of the most principal analytical technique for mapping analysis for major to trace elements in rocks, minerals, functional materials, or biological tissue samples. In this study, imaging analysis was conducted with coupling of small volume cell and off-set laser ablation protocol to improve the spatial resolution. Combination of newly designed small volume cell and in-torch gas mixing protocols provides faster washout time of the signals (about 0.8 s for reducing238U being one part in a hundred, 1% level). This is very important to improve the spatial resolution in a direction of laser scanning. Moreover, combination of small distances between the laser-line scan (laser pitch distance) and preferential and total ablation of only biological tissue samples placed on glass substrate results in laser ablation of smaller areas than the size of laser ablation pit (shaving ablation). With the shaving ablation, laser-line scanning with narrower-band width (e.g., 2 µm) can be achieved even by the laser beam of 8 µm diameter. To demonstrate the practical usage of the present technique, imaging analysis of Gd-ethylenediamine tetra-methylene phosphonic acid-doped mouse bone was conducted. Preferential distribution of Gd at the edge of the apatite cell was more clearly identified by the present technique. Combination of the shorter washout system setup and the shaving ablation protocol enables us to improve the spatial resolution of the elemental imaging obtained with the LA-ICPMS technique.Graphical abstract