Measurement of 8-Iso-Prostaglandin F2α in Biological Fluids as a Measure of Lipid Peroxidation
Measurement of 8-Iso-Prostaglandin F2α in Biological Fluids as a Measure of Lipid Peroxidation
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DOI：
10.1007/978-1-59745-364-6_14
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发表时间：
2010-01-01
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影响因子：
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通讯作者：
Patrignani, Paola
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文献类型：
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作者：
Tacconelli, Stefania;Capone, Marta L.;Patrignani, Paola
一些证据表明，活性氧与人类疾病有关，包括动脉粥样硬化、高血压和血管成形术后再狭窄。F-2-异前列烷（F-2-iPs），形成非酶通过自由基催化攻击酯化花生四烯酸酯的测量，提供了一个可靠的工具，用于识别人口的脂质过氧化率提高。在F-2-isoP中，8-iso-PGF（2 α）（也称为IPF 2（2 α）-III）和IPF 2 α-VI是生物体液中最常测量的。已经提出了多种方法来测量尿液和血浆中的F-2-异前列烷。已经开发了用于测量F-2-异前列烷的质谱法，但其使用受到限制，因为其耗时且非常昂贵。我们已经开发了经验证的酶免疫测定（EIA）和放射免疫测定（RIA）技术，使用高度特异性的抗血清来测量8-iso-PGF（2 α）。相反，商业上可获得的免疫测定试剂盒因其低特异性而受到限制。尿中特异性异前列腺素（如8-iso-PGF（2 α））的测量是一种可靠的、非侵入性的脂质过氧化指标，对天然和合成抗氧化剂的剂量确定研究有价值的帮助。
Several lines of evidence suggest that reactive oxygen species are implicated in human disease, including atherosclerosis, hypertension, and restenosis after angioplasty. The measurement of F-2-isoprostanes (F-2-iPs), formed nonenzymatically through free radical catalyzed attack on esterified arachidonate, provides a reliable tool for identifying populations with enhanced rates of lipid peroxidation. Among F-2-isoPs, 8-iso-PGF(2 alpha) (also referred to and IPF2(2 alpha)-III) and IPF2 alpha-VI are the most frequently measured in biological fluids. A variety of methods have been proposed to measure F-2-isoprostanes in urine and plasma. Mass spectrometry has been developed for the measurement of both F-2-isoprostanes but its use is limited as it is time-consuming and highly expensive. We have developed validated enzyme immunoassay (EIA) and radioimmunoassay (RIA) techniques using highly specific antisera for the measurement of 8-iso-PGF(2 alpha) In contrast, the commercially available immunoassay kits are limited for their poor specificity. The measurement of specific isoprostanes, such as 8-iso-PGF(2 alpha), in urine is a reliable, noninvasive index of lipid peroxidation that is of valuable help in dose-finding studies of natural and synthetic antioxidant agents.