Isolation, analysis of structure, synthesis, and biological actions of urotensin I neuropeptides.
Isolation, analysis of structure, synthesis, and biological actions of urotensin I neuropeptides.
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尾加压素 I 神经肽的分离、结构分析、合成和生物学作用。
DOI：
10.1139/o83-076
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Fryer,J
中科院分区：
文献类型：
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作者：
Lederis,K;Letter,A;McMaster,D;Ichikawa,T;MacCannell,KL;Kobayashi,Y;Rivier,J;Rivier,C;Vale,W;Fryer,J
从两种硬骨鱼(鲤鱼和商业硬骨鱼)的尾部分离和纯化了41个残基的神经肽尾加压素I(UI)，并测定了其氨基酸序列，通过合成全活性多肽对其进行了确认。该多肽与绵羊下丘脑促肾上腺皮质激素释放因子(CRF)和青蛙皮肤多肽苏云金具有密切的结构和生物同源关系，是这类多肽的系统发育原型。在热醋酸或盐酸中提取尿赘，裂解氨基末端三肽，产生完全活性的UI(4-41)。在释放哺乳动物促肾上腺皮质激素(ACTH)方面，UI与其他两种天然多肽(CRF和苏维林)相当，但在刺激鱼类脑垂体ACTH释放方面，UI比哺乳动物同源物强几倍。UI肽及其哺乳动物或两栖同系物在哺乳动物中具有持久的降压作用，仅通过在肠系膜上(前)血管床上选择性地扩张血管。哺乳动物同系物(CRF)显著降低血压的血管扩张作用表明，在从鱼类到哺乳动物的系统发育过程中，哺乳动物胃肠道中的UI肽的血流动力学作用的未知生理作用发生了变化。
The 41-residue neuropeptide urotensin I (UI), from the urophyses of two teleost fish species (Cyprinus carpioandCatostomus commersoni), was isolated and purified, and its amino acid sequence was determined and confirmed by synthesis of a fully active peptide. The UI peptide was found to be a close structural and biological homologue of the ovine hypothalamic corticotropin-releasing factor (CRF) and the frog skin peptide sauvagine; UI is, therefore, a phylogenetic prototype of this group of peptides. Extraction of urophyses in hot acetic or hydrochloric acid cleaves an amino terminal tripeptide yielding a fully active UI(4–41). The UI peptides are equipotent with the other two naturally occurring peptides (CRF and sauvagine) in the release of mammalian pituitary corticotropin (ACTH), but UI is several times more potent than the mammalian homologue in the stimulation of release of fish pituitary ACTH. The UI peptide and its mammalian or amphibian homologues have a long-lasting hypotensive action in mammals, via a uniquely selective vasodilatation in the superior (anterior) mesenteric vascular bed only. The significantly lower hypotensive vasodilatory action of the mammalian homologue (CRF) suggests a change in the unknown physiological role of the haemodynamic actions of the UI peptides in the mammalian gastrointestinal tract during phylogenetic progression from fishes to mammals.