Spatial Characterization of the Human Centrosome Proteome Opens Up New Horizons for a Small but Versatile Organelle
Spatial Characterization of the Human Centrosome Proteome Opens Up New Horizons for a Small but Versatile Organelle
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DOI：
10.1002/pmic.201900361
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发表时间：
2020-08-26
期刊：
影响因子：
3.4
通讯作者：
Lundberg, Emma
中科院分区：
文献类型：
--
作者：
Danielsson, Frida;Mahdessian, Diana;Lundberg, Emma
经过一个世纪的研究，人类中心体仍然令人着迷。基于免疫荧光和共聚焦显微镜，提供了人类中心体和中心粒卫星的蛋白质成分的广泛清单，其中 300 多种新蛋白质定位于这些区室的重要贡献。确定了参与泛素化的候选中心体蛋白网络，包括 Kelch 样蛋白 21 的 6 个相互作用伙伴，以及一个额外的蛋白磷酸酶网络，共同支持中心体作为细胞信号传导交互中心的作用。人类蛋白质图谱 (HPA) 项目中对跨细胞器的多定位分析表明，多定位蛋白质在中心粒卫星中的比例特别高，支持了该区室的动态性质和广泛的功能。总之，此处描述的人类中心体和中心粒卫星的空间解剖为进一步探索其蛋白质组提供了全面的知识库。
After a century of research, the human centrosome continues to fascinate. Based on immunofluorescence and confocal microscopy, an extensive inventory of the protein components of the human centrosome, and the centriolar satellites, with the important contribution of over 300 novel proteins localizing to these compartments is presented. A network of candidate centrosome proteins involved in ubiquitination, including six interaction partners of the Kelch-like protein 21, and an additional network of protein phosphatases, together supporting the suggested role of the centrosome as an interactive hub for cell signaling, is identified. Analysis of multi-localization across cellular organelles analyzed within the Human Protein Atlas (HPA) project shows how multi-localizing proteins are particularly overrepresented in centriolar satellites, supporting the dynamic nature and wide range of functions for this compartment. In summary, the spatial dissection of the human centrosome and centriolar satellites described here provides a comprehensive knowledgebase for further exploration of their proteomes.