Transplantation of amniotic membrane to the subretinal space in pigs.
Transplantation of amniotic membrane to the subretinal space in pigs.
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DOI：
10.1155/2012/716968
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发表时间：
2012
影响因子：
4.3
通讯作者：
la Cour M
中科院分区：
文献类型：
--
作者：
Kiilgaard JF;Scherfig E;Prause JU;la Cour M
目的.目的探讨羊膜移植对视网膜下创伤愈合的影响。方法. 9只丹麦长白猪进行了视网膜色素上皮（RPE）的手术切除和布鲁赫膜（BM）的机械损伤，并作为对照组。另外15只猪将AM移植到视网膜下腔。结果当获得诱导缺陷的完全覆盖时，AM显著减少脉络膜新生血管形成（7只猪）（P < 0.05）。在AM未覆盖BM破裂的情况下，脉络膜组织覆盖移植膜（8只猪）。AM在视网膜下腔中耐受良好，仅引起有限的炎症，并且当与宿主RPE接触时被单层色素细胞覆盖。结论. AM改变BM损伤后的脉络膜新生血管形成，并可作为RPE的基底膜替代物。
Purpose. To investigate the effect of transplanted amniotic membrane (AM) on subretinal wound healing. Methods. Nine Danish Landrace pigs had surgical removal of retinal pigment epithelium (RPE) and mechanical damage of Bruch's membrane (BM) and served as a control group. 15 pigs additionally had AM transplanted to the subretinal space. Results. AM significantly reduces choroidal neovascularisation when complete coverage of the induced defect is obtained (7 pigs) (P < 0.05). In cases where AM did not cover the rupture in BM choroidal tissue covered the transplanted membrane (8 pigs). AM is well tolerated in the subretinal space, causes only limited inflammation, and is covered with a monolayer of pigmented cells when in contact with the host RPE. Conclusions. AM modifies choroidal neovascularisation after BM damage and may serve as a basement membrane substitute for the RPE.