A Microfluidic Device for Presumptive Testing of Controlled Substances *
A Microfluidic Device for Presumptive Testing of Controlled Substances *
复制标题
用于受控物质推定测试的微流体装置*
DOI：
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发表时间：
2007
影响因子：
1.6
通讯作者：
Rebecca D. Hanes
中科院分区：
文献类型：
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作者：
S. Bell;Rebecca D. Hanes
翻译后摘要：一个简单的微流控装置（MFD）已被开发用于执行多种颜色和晶体测试的受控物质分析。 MFD方法使用更少的样品和试剂，比传统的点板方法产生更少的废物，同时进行多个测试。这种方法为单样品分析提供了更多的分析信息。目前的设备是一个显微镜载玻片的大小，有四个分析通道：一个用于微晶测试，三个用于颜色测试。使用比较重复分析和几名操作员对优化的器械进行了严格的验证研究。目标分析物为甲基苯丙胺、苯丙胺、可卡因和羟考酮，使用的颜色检测试剂为Marquis、Simon和硫氰酸钴。对于晶体测试，使用氯化铂。验证研究表明，MFD的检测限在皮克范围内。在受控物质浓度为5-10%（w/w）的复杂混合物中观察到阳性试验结果。当用于该装置时，微晶试剂显示出比颜色测试试剂更高的灵敏度。使用该设备的试剂使用和废物产生比使用传统方法减少95%。器械性能也显示与操作员无关。
Abstract: A simple microfluidic device (MFD) has been developed to perform multiple color and crystal tests for controlled substance analysis. The MFD method uses less sample and reagents and generates less waste than traditional spot plate methods while performing several tests simultaneously. This methodology provides significantly more analytical information for a single sample analysis. The current generation device is the size of a microscope slide with four analytical channels: one for microcrystal tests and three for color tests. The optimized devices were subjected to a rigorous validation study using comparative replicate analyses and several operators. Target analytes were methamphetamine, amphetamine, cocaine, and oxycodone and color test reagents used were the Marquis, Simon, and cobalt thiocyanate. For the crystal tests, platinic chloride was used. The validation study showed the MFD’s limits of detection to be in the picogram range. Positive tests results were observed in complex mixtures in which the controlled substance was present at concentrations of 5–10% (w/w). The microcrystal reagents showed greater sensitivity than color test reagents when used in the device. Reagent use and waste generation using the devices was 95% less that that used and generated using the traditional methods. The device performance was also shown to be operator independent.