An active DNA transposon nDart causing leaf variegation and mutable dwarfism and its related elements in rice
An active DNA transposon nDart causing leaf variegation and mutable dwarfism and its related elements in rice
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DOI：
10.1111/j.1365-313x.2005.02600.x
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发表时间：
2006-01-01
期刊：
影响因子：
7.2
通讯作者：
Iida, S
中科院分区：
文献类型：
--
作者：
Tsugane, K;Maekawa, M;Iida, S
自从 Barbara McClintock 发现解离赋予杂色以来，由 DNA 转座子引起的特征性可变等位基因在玉米中非常丰富，但在水稻中只描述了少数可变等位基因，尽管水稻基因组包含各种转座子。在这里，我们发现自发突变的绿色等位基因 pyl-v 是由核编码的必需叶绿体蛋白酶基因 OsClpP5 的破坏引起的，这是由于插入了一个 607 bp 的非自主 DNA 转座子，即属于 hAT 超家族的基于非自主 DNA 的活性水稻转座子 1 (nDart1)。 nDart1 的转座可以通过与含有自主元件 aDart 的品系杂交来诱导，并通过从 aDart 中分离出来来稳定。我们还发现了一个由 nDart1 插入引起的新的可变矮等位基因 thl-m。粳稻品种 Nipponbare 不携带 aDart，尽管它含有表观遗传沉默的 Dart 元件，可被 5-氮杂胞苷激活。日本晴具有四个约 3.6 kb 的 Dart 样序列亚组，其中三个包含潜在的转座酶基因，以及约 3.6 kb 的元件，不含明显的转座酶基因，以及三个约 0.6 kb 的 nDart1 相关元件亚组，这些元件都是 Dart 样序列的内部缺失。 nDart1 和 3.6-kb Dart 样元素也存在于籼稻品种 93-11 和 Kasalath 中。 nDart1 似乎是 nDart1 相关元件中最活跃的诱变剂，有助于产生自然变异。讨论了自主元件 aDart 的候选者以及 nDart1 在转座子标记方面的可能应用。
While characterized mutable alleles caused by DNA transposons have been abundant in maize since the discovery of Dissociation conferring variegation by Barbara McClintock, only a few mutable alleles have been described in rice even though the rice genome contains various transposons. Here, we show that a spontaneous mutable virescent allele, pyl-v, is caused by the disruption of the nuclear-coded essential chloroplast protease gene, OsClpP5, due to insertion of a 607-bp non-autonomous DNA transposon, non-autonomous DNA-based active rice transposon one (nDart1), belonging to the hAT superfamily. The transposition of nDart1 can be induced by crossing with a line containing an autonomous element, aDart, and stabilized by segregating out of aDart. We also identified a novel mutable dwarf allele thl-m caused by an insertion of nDart1. The japonica cultivar Nipponbare carries no aDart, although it contains epigenetically silenced Dart element(s), which can be activated by 5-azacytidine. Nipponbare bears four subgroups of about 3.6-kb Dart-like sequences, three of which contain potential transposase genes, and around 3.6-kb elements without an apparent transposase gene, as well as three subgroups of about 0.6-kb nDart1-related elements that are all internal deletions of the Dart-like sequences. Both nDart1and 3.6-kb Dart-like elements were also present in indica varieties 93-11 and Kasalath. nDart1 appears to be the most active mutagen among nDart1-related elements contributing to generating natural variations. A candidate for an autonomous element, aDart, and a possible application of nDart1 for transposon tagging are discussed.