Analysis of microorganisms with nonlinear electrokinetic microsystems
Analysis of microorganisms with nonlinear electrokinetic microsystems
复制标题
利用非线性电动微系统分析微生物
DOI：
10.1002/elps.202000233
复制
发表时间：
2021
期刊：
影响因子：
2.9
通讯作者：
Lapizco‐Encinas, Blanca H.
中科院分区：
文献类型：
--
作者：
Hakim, Kel S.;Lapizco‐Encinas, Blanca H.
非线性电动力学（EK），特别是第二类电泳，dielectrophoresis （DEP）和electroorotion (EROT)，由于其灵活性和无标签的操作方式而引起了人们的极大兴趣。这些技术目前的应用与它们最初被发现时相比有了明显的进步，但仍显示出未来增长的强劲迹象。本文综述了目前微尺度非线性EK技术作为微生物分析、传感和纯化工具的应用。讨论的重点是各种非线性EK微流控技术的一些最新发现，如用于颗粒评估的DEP颗粒捕获和EROT，用于分析从病毒到寄生虫的微生物。在此过程中，特别关注了过去两年内的关键研究文章，以提供有关微尺度EK领域当前技术状况的最新知识，并从那里展望了该领域的未来。
Nonlinear electrokinetics (EK), specifically electrophoresis of the second kind, dielectrophoresis (DEP) and electrorotation (EROT), have gained significant interest recently for their flexibility and labeless discriminant manner of operation. The current applications of these technologies are a clear advancement from what they were when first discovered, but also still show strong signs of future growth. The present review article presents a discussion of the current uses of microscale nonlinear EK technologies as analytical, sensing, and purification tools for microorganisms. The discussion is focused on some of the latest discoveries with various nonlinear EK microfluidic techniques, such as DEP particle trapping and EROT for particle assessments, for the analysis of microorganisms ranging from viruses to parasites. Along the way, special focus was given to key research articles from within the past two years to provide the most up‐to‐date knowledge on the current state‐of‐the‐art within the field of microscale EK, and from there, an outlook on where the future of the field is headed is also included.