The hypothalamic suprachiasmatic nucleus of rat: intrinsic anatomy.
The hypothalamic suprachiasmatic nucleus of rat: intrinsic anatomy.
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大鼠下丘脑视交叉上核：内在解剖学。
DOI：
10.1002/cne.901910410
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
VandenPol,AN
中科院分区：
文献类型：
--
作者：
VandenPol,AN
采用高尔基浸渍法、尼氏染色法、辣根过氧化物酶染色法和电镜等方法，对数百只大鼠脑视交叉上核（SCN）的内部结构进行了定性和定量研究。一个视交叉上核的体积约为0.068 mm3，包含近8000个神经元。背内侧，细胞比腹侧更小，排列更紧密；与细胞核的其他部分相比，后内侧SCN的体细胞配对明显更多。具有扩展的体细胞相邻区域的两个神经元可能没有细胞间特化，或者它们可能通过各种附着斑块结合在一起。尼氏染色和银染色以及电镜显示，在SCN的背内侧发现了长区域体体相关的神经元链。这些链的长度通常是前后方向的。星形胶质细胞散布在神经元中。用高尔基浸渍、Cajal’s金升华染色和电镜研究SCN中的星形胶质细胞可能具有丰富的细胞质，介于一些大神经元的富含细胞器的细胞质和一些较小的SCN神经元的细胞器缺乏细胞质之间。SCN胶质细胞和神经元的细胞核常内陷，多核仁是大量SCN神经元的显著特征。高尔基浸渍会产生一些相对简单的树突状乔木。这些细胞包括简单双极细胞、卷曲双极细胞、放射状神经元、单极神经元和棘细胞。在核的边缘，一些树突可以进入邻近的下丘脑前部；同样，来自SCN外细胞的树突也可能进入细胞核边界。与下丘脑的其余部分相比，SCN轴突用传统的组织学方法（包括Lux01 blue、Bodian、Sevier-Munger、Loyez和Golgi）染色效果很差，部分原因是纤维的细口径。高尔基浸渍和银染的轴突束主要由无髓鞘轴突组成，可在细胞核内分裂或通过而不保留局部侧支。几种不同类型的高尔基浸渍轴突在SCN内终止。有些在SCN体细胞、树突和树突附属物上有大量的钮扣。其他单轴突穿过SCN时没有在细胞核中留下任何侧支或终末。大多数由SCN神经元产生的高尔基浸染轴突维持局部终止侧支，具有过路和终末的扣；这些轴突起源于核周或近端树突的频率相同。高尔基、辣根过氧化物酶和银染色显示轴突连接左右SCN。SCN是一个复杂的细胞核，具有可识别的细分，其中包含几种类型相对简单的树突乔木的神经元。在SCN的任何区域内，可以发现相邻细胞之间的超微结构差异，表明神经元的异质性。结合本研究结果和先前报道的数据，SCN的神经元具有大量的
The internal structure of the suprachiasmatic nucleus (SCN) was studied qualitatively and quantitatively in several hundred rat brains with a variety of methods including several types of Golgi impregnations, Nissl stains, horseradish peroxidase application, and electron microscopy. One suprachiasmatic nucleus has a volume of about 0.068 mm3 and contains close to 8,000 neurons. Dorsomedially, cells tend to be smaller and more tightly packed than ventrolaterally; significantly more somatic appositions occur in the dorsomedial SCN than in other parts of the nucleus. Two neurons with an extended region of somatic apposition may have no intercellular specializations, or they may be held together with various attachment plaques. Chains of neurons with long regions of somatosomatic apposition are found in the dorsomedial SCN with Nissl and silver stains, and with EM. The length of these chains is generally oriented in an antero-posterior direction. Interspersed with the neurons are astroglia. Astroglia studied with Golgi impregnations, Cajal's gold sublimate stain, and EM in SCN may have a rich cytoplasm falling in between the organelle-rich cytoplasm of some large neurons and the organelle-poor cytoplasm of some of the smaller SCN neurons. Nuclei of SCN glia and neurons are often invaginated and multiple nucleoli are a prominent feature of a large number of SCN neurons. With Golgi impregnations a number of relatively simple dendritic arbors exist. These include the simple bipolar cell, curly bipolar cell, radial neuron, monopolar neuron, and spinous cell. At the borders of the nucleus some dendrites may travel into the adjacent anterior hypothalamus; similarly, dendrites from cells outside SCN may enter into the nucleus boundaries. Compared with the rest of the hypothalamus, axons in SCN stain very poorly with conventional histological methods including Lux01 blue, Bodian, Sevier-Munger, Loyez, and Golgi, in part because of the fine caliber of the fibers. Golgi impregnated and silver-stained axon fascicles composed primarily of unmyelinated axons may divide up within the nucleus or may pass through without maintaining local collaterals. Several different types of Golgi-impregnated axonal arborizations terminate within SCN. Some have an extensive number of boutons ending on SCN somata, dendrites, and dendritic appendages. Other single axons pass through SCN without leaving any collaterals, or terminals in the nucleus. The majority of Golgi-impregnated axons arising from SCN neurons maintain locally terminating collaterals, with boutons en passant and termineaux; these axons originate with equal frequency from the perikaryon or from a proximal dendrite. Golgi, horseradish peroxidase, and silver staining methods reveal axons connecting left and right SCN.The SCN is a complex nucleus with recognizable subdivisions that contain neurons with several types of relatively simple dendritic arbors. Within any area of SCN, ultrastructural differences can be found between neighboring cells, suggesting a heterogenous population of neurons. Combining the results of the present study with previously reported data, neurons of the SCN have a large number of