Paenibacillus pectinilyticus sp. nov., isolated from the gut of Diestrammena apicalis.
Paenibacillus pectinilyticus sp. nov., isolated from the gut of Diestrammena apicalis.
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DOI：
10.1099/ijs.0.002261-0
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发表时间：
2009-06
影响因子：
2.8
通讯作者：
Doo-Sang Park;W. Jeong;K. Lee;H. Oh;Byung-Chun Kim;K. Bae;Ho‐Yong Park
中科院分区：
文献类型：
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作者：
Doo-Sang Park;W. Jeong;K. Lee;H. Oh;Byung-Chun Kim;K. Bae;Ho‐Yong Park
在昆虫肠道中寻找产生外酶的细菌时，一种能够降解果胶的新型细菌被分离出来。该菌株命名为RCB-08(T)，由革兰氏阳性，内孢子形成，可在15-30℃和pH 6.0-8.7下生长的可运动棒组成。该菌株DNA G+C含量为51.5 mol%，主要细胞脂肪酸为前异丙酸（15.0）（74.1%）。基于16S rRNA基因序列的系统发育分析表明，菌株RCB-08(T)隶属于Paenibacillus chondroitinus NBRC 15376(T)，与Paenibacillus chondroitinus NBRC 15376(T)亲缘关系最密切，序列相似性为96.7%。菌株RCB-08(T)与P. chondroitinus NBRC 15376(T)的DNA-DNA亲缘度为15.0%。菌株RCB-08(T)能水解果胶，但不能水解纤维素、酪蛋白、淀粉和木聚糖。菌株RCB-08(T)可以通过多相法观察到的特征与其他类芽孢杆菌明显区分开。因此，菌株RCB-08(T)被认为代表了Paenibacillus属的一个新种，并建议将其命名为Paenibacillus pectinilyticus sp. 11。型应变为RCB-08(T) (=KCTC 13222（T)=CECT 7358(T)）。
During a search for exo-enzyme-producing bacteria in the gut of an insect, Diestrammena apicalis, a novel bacterium capable of degrading pectin was isolated. The isolate, designated strain RCB-08(T), comprised Gram-positive, endospore-forming, motile rods capable of growth at 15-30 degrees C and pH 6.0-8.7. The DNA G+C content of the isolate was 51.5 mol% and the predominant cellular fatty acid was anteiso-C(15 : 0) (74.1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RCB-08(T) was affiliated with a cluster within the Paenibacillaceae, and was related most closely to Paenibacillus chondroitinus NBRC 15376(T), with a sequence similarity of 96.7 %. The DNA-DNA relatedness value for strain RCB-08(T) with P. chondroitinus NBRC 15376(T) was 15.0 %. Strain RCB-08(T) hydrolysed pectin, but not cellulose, casein, starch or xylan. Strain RCB-08(T) could be clearly distinguished from other Paenibacillus species on the basis of characteristics observed using a polyphasic approach. Therefore strain RCB-08(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus pectinilyticus sp. nov. is proposed. The type strain is RCB-08(T) (=KCTC 13222(T)=CECT 7358(T)).