Overexpression of mango MiMFT inhibits seed germination and enhances abiotic stress tolerance in transgenic Arabidopsis
Overexpression of mango MiMFT inhibits seed germination and enhances abiotic stress tolerance in transgenic Arabidopsis
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DOI：
10.1016/j.scienta.2022.111495
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发表时间：
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影响因子：
4.3
通讯作者：
Ting-ting Lu;Zhi-yi Fan;Xinhua He;Hai-xia Yu;Ronghe Liang;Xing Huang;Yi-li Zhang;Jia-wei Zhu-Jia-wei
中科院分区：
文献类型：
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作者：
Ting-ting Lu;Zhi-yi Fan;Xinhua He;Hai-xia Yu;Ronghe Liang;Xing Huang;Yi-li Zhang;Jia-wei Zhu-Jia-wei
FT和TFL1的母亲(MFT)是磷脂酰乙醇胺结合蛋白(PEBP)家族的成员，在种子发育、逆境反应和开花时间调控中发挥重要作用。在本研究中，在芒果品种‘四季蜜’中发现了MF同源基因MiMFT。MiMFT的启动子含有植物激素、植物生长、植物发育和非生物胁迫反应元件，MiMFT在种子中显著高表达，并且在聚乙二醇(PEG)和氯化钠处理下表达强烈增强。在拟南芥中过表达MiMFT对种子萌发有不利影响，并上调了种子萌发相关基因的表达，但不影响开花。此外，过量表达MiMFT的转基因拟南芥的耐盐性和抗旱性显著提高，这些植株对脱落酸(ABA)的敏感性降低，胁迫相关基因和ABA信号通路基因的表达显著增加。亚细胞定位分析表明MiMFT强烈定位于细胞核，双分子荧光互补(BIFC)分析表明MiMFT蛋白与Mi14-3-3D2相互作用。综上所述，MiMFT被确定参与种子萌发以及对非生物胁迫的反应。
Mother of FT and TFL1(MFT) is a member of the phosphatidylethanolamine-binding protein (PEBP) family, which plays important roles in seed development, the stress response, and flowering time regulation. In the present study, theMFThomologous geneMiMFTwas identified in mango cultivar ‘SiJiMi’. The promoter ofMiMFTcontained phytohormone-, plant growth-, plant development-, and abiotic stress-responsive elements.MiMFTwas significantly highly expressed in the seeds, and its expression strongly increased in response to polyethylene glycol (PEG) and NaCl treatment. Overexpression ofMiMFTinArabidopsisadversely affected seed germination and upregulated seed germination-related gene expression, but it did not affect flowering. Additionally, the salt and drought tolerance of transgenicArabidopsislines overexpressingMiMFTconsiderably increased, and these plants presented decreased sensitivity to abscisic acid (ABA), with significantly increased expression of stress-related and ABA signaling pathway genes. Subcellular localization analysis showed that MiMFT strongly localizes in the nucleus, and bimolecular fluorescence complementation (BiFC) assays showed that MiMFT proteins interact with Mi14–3–3D2. In summary,MiMFTwas determined to be involved in seed germination as well as in the response to abiotic stress.