Expression of m-Golsyn/Syntabulin gene during mouse brain development
Expression of m-Golsyn/Syntabulin gene during mouse brain development
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DOI：
10.1016/j.neulet.2006.04.061
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发表时间：
2006-08-07
影响因子：
2.5
通讯作者：
Ito, Fumiaki
中科院分区：
文献类型：
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作者：
Funakoshi, Eishi;Fukui, Masaki;Ito, Fumiaki
我们最近分离出小鼠Golsyn/Syniabulin（m-Golsyn/Syntabulin）基因的cDNA，并将其定位于小鼠染色体15B3.2与人类染色体8 q23同线，在该染色体上定位了一个负责原发性开角型青光眼的位点。本研究利用抗GOLSYN抗体检测了小鼠脑内不同区域m-Golsyn/Syntabulin蛋白的表达及其发育变化。m-Golsyn/Syntabulin蛋白在胚胎第14天和整个出生后阶段的不同脑区中检测到。此外，随着脑组织发生和成熟的进行，在脉络丛、梨状皮质、锥体细胞层和浦肯野细胞层的细胞中可检测到该蛋白的强表达。小鼠脑的原位杂交分析显示，m-Golsyn/Syntabulin转录物的定位与m-Golsyn/Syntabulin蛋白的定位非常相似，证实了m-Golsyn/Syntabulin基因在特定脑区域中的高水平表达。在包括睫状体在内的眼组织中也观察到m-Golsyn/Syntabulin蛋白的高水平表达，睫状体是已知的产生房水的部位。这些结果可能表明这种蛋白质在神经元细胞和其他类型的细胞，如脉络丛和睫状体的细胞中的重要作用。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
We recently isolated the cDNA for the mouse Golsyn/Syniabulin (m-Golsyn/Syntabulin) gene and mapped it to mouse chromosome 15B3.2 syntenic with human chromosome 8q23, on which a locus responsible for primary open-angle glaucoma had been located. In the present study, we examined the expression of m-Golsyn/Syntabulin protein in various regions of mouse brain and its developmental changes by use of anti-GOLSYN antibody. m-Golsyn/Syntabulin protein was detected in various brain regions at embryonic day 14 and throughout the postnatal stages. Furthermore, as the histogenesis and maturation of brain proceeded, strong expression of the protein became detectable in cells of the choroid plexus, piriform cortex, pyramidal cell layer, and Purkinje cell layer. In situ hybridization analysis of the mouse brain revealed that localization of the m-Golsyn/Syntabulin transcript was very similar to that of m-Golsyn/Syntabulin protein, confirming the high-level expression of the m-Golsyn/Syntabulin gene in the specific brain regions. High-level expression of m-Golsyn/Syntabulin protein was also observed in the ocular tissues including the ciliary body, which is known as a site for the production of aqueous humor. These results may indicate a significant role for this protein in neuronal cells and other types of cells such as those of the choroid plexus and ciliary body. (c) 2006 Elsevier Ireland Ltd. All rights reserved.