Peroxiredoxin is ubiquitously expressed in rat skin: Isotype-specific expression in the epidermis and hair follicle
Peroxiredoxin is ubiquitously expressed in rat skin: Isotype-specific expression in the epidermis and hair follicle
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DOI：
10.1046/j.1523-1747.2000.00177.x
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发表时间：
2000-12-01
影响因子：
6.5
通讯作者：
Kim, YP
中科院分区：
文献类型：
--
作者：
Lee, SC;Chae, HZ;Kim, YP
过氧化物还原酶是一个在哺乳动物组织中广泛表达的过氧化物酶家族，然而，对其在皮肤中的表达知之甚少。在这项研究中，我们研究了三种同型过氧化物酶（I-III）在大鼠皮肤中的表达。蛋白质印迹分析表明，在完整皮肤的表皮和真皮中过氧化物酶I-III的强表达。此外，它们在培养的大鼠角质形成细胞和成纤维细胞中表达。共聚焦图像分析显示，peroxiredoxin II存在于细胞质中作为一个弥漫性的，网状图案。在大鼠皮肤的免疫组化染色中，过氧化物酶的表达主要定位于表皮、毛囊和皮脂腺。在表皮中，过氧化物酶I和II在所有层中表达，表达梯度增加到颗粒层。与此相反，过氧化物氧还蛋白III在所有层中表达，表达梯度向颗粒层降低。在毛囊中，过氧化物酶I-III主要表达在外根鞘，除了过氧化物酶II，这是强烈表达在内根鞘。原位杂交结果显示，mRNA表达与蛋白水平是相称的。紫外线B照射后15 min内增加大鼠皮肤中过氧化物氧还蛋白II的表达。从这项研究中，我们得出结论，过氧化物氧还蛋白亚型普遍表达在大鼠皮肤中，至少过氧化物氧还蛋白II的表达可以调节紫外线照射作为过氧化物酶在皮肤中。
Peroxiredoxins are a family of peroxidases that are ubiquitously and abundantly expressed in mammalian tissues; however, comparatively less is known about their expression in the skin. In this study, we examined the expression of three isotypes of peroxiredoxins (I-III) in rat skin. Western blot analyses showed strong expression of peroxiredoxins I-III in the epidermis and dermis of intact skin. Additionally, they were expressed in cultured rat keratinocytes and fibroblasts. Confocal image analyses revealed that peroxiredoxin II was present in the cytoplasm as a diffuse, reticulated pattern. In immunohistochemical staining of rat skin, peroxiredoxin expression was mainly localized to the epidermis, hair follicles, and sebaceous glands. In the epidermis, peroxiredoxins I and II were expressed in all layers with a gradient of increasing expression to the granular layer. In contrast, peroxiredoxin III was expressed in all layers with a gradient of expression decreasing to the granular layer. In the hair follicle, peroxiredoxins I-III were mainly expressed in the outer root sheath, except peroxiredoxin II, which was strongly expressed in the inner root sheath. In situ hybridization showed that mRNA expression was commensurate with the level of protein. Ultraviolet B radiation increased peroxiredoxin II expression in rat skin within 15 min after irradiation. From this study we conclude that peroxiredoxin isoforms are ubiquitously expressed in rat skin, and expression of at least peroxiredoxin II can be regulated by ultraviolet irradiation as a peroxidase in the skin.