HongrES1, a Cauda Epididymis-Specific Protein, Is Involved in Capacitation of Guinea Pig Sperm
HongrES1, a Cauda Epididymis-Specific Protein, Is Involved in Capacitation of Guinea Pig Sperm
复制标题
HongrES1，一种附睾尾特异性蛋白，参与豚鼠精子获能
DOI：
10.1002/mrd.21063
复制
发表时间：
2009-10-01
影响因子：
2.5
通讯作者：
Shi, Qi-Xian
中科院分区：
文献类型：
--
作者：
Ni, Ya;Zhou, Yuchuan;Shi, Qi-Xian
获能需要去除附睾尾分泌的蛋白质。在此之前，我们从大鼠附睾尾分离并克隆了HongrES 1基因，发现它只在那里表达。在这里，我们报告的HongrES 1 mRNA也表达在豚鼠附睾尾使用北方印迹分析，其同源蛋白的分子量约为48 kDa的Western印迹分析。因此，我们研究了HongrES 1是否参与了豚鼠精子获能的调节。结果表明，HongrES 1抗血清（HA）在1：500稀释度下显著增强精子获能，具有最大刺激。当获能的精子再次暴露于HongrES 1蛋白（HP，0.25 μ g/ml）时，获能被逆转。换句话说，惠普充当了一个断头因素。HA加速获能的发生并促进精子超活化运动反应。HA刺激细胞外钙离子内流可加速精子获能，而HIP则抑制细胞外钙离子内流。间接免疫荧光染色发现HP定位于精子头部顶体前部，在获能培养过程中逐渐脱落，顶体反应后完全消失。因此，由附睾尾表达的HongrES 1是一种在受精前调节豚鼠精子生理学的新分子。
Capacitation requires removal of proteins secreted by the cauda epididymis. Previously, we isolated and cloned the HongrES1 gene from rat cauda epididymis and found that it was exclusively expressed there. Here we report that HongrES1 mRNA is also expressed in the guinea pig cauda epididymis using Northern blot analysis, and the molecular weight of its cognate protein is approximately 48 kDa by Western blot analysis. Therefore, we investigated whether HongrES1 was involved in regulation of sperm capacitation in guinea pig. The results show that HongrES1 antisera (HA) significantly enhances sperm capacitation with maximal stimulation at a dilution of 1:500. Capacitation was reversed when capacitated spermatozoa were re-exposed to HongrES1 protein (HP, 0.25 mu g/ml). In other words, HP acted as a decapacitation factor. HA accelerated the onset of capacitation and promoted a sperm hyperactivated motility response. Sperm capacitation was accelerated by HA stimulation of extracellular calcium influx while HIP prevented extracellular calcium from influxing. Indirect immunofluorescence staining finds HP localized over the acrosomal anterior region of the sperm head, which exfoliates gradually during capacitation incubation, and completely disappeared after the acrosome reaction. Thus, HongrES1 expressed by the cauda epididymis is a novel molecule that regulates the physiology of guinea pig sperm prior to fertilization.