Effect of antioxidant-enriched diets on glutathione redox status in tissue homogenates and mitochondria of the senescence-accelerated mouse
Effect of antioxidant-enriched diets on glutathione redox status in tissue homogenates and mitochondria of the senescence-accelerated mouse
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DOI：
10.1016/j.freeradbiomed.2005.04.008
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发表时间：
2005-08-15
影响因子：
7.4
通讯作者：
Sohal, RS
中科院分区：
文献类型：
--
作者：
Rebrin, I;Zicker, S;Sohal, RS
这项研究的主要目的是调查食用富含抗氧化剂的饮食是否可以减轻加速衰老小鼠(SAM)的氧化应激水平。在单独和独立的研究中，两种不同的日粮混合物，一种富含维生素E、维生素C、L-肉碱和硫辛酸的日粮(日粮1)和另一种包括维生素E和C的日粮以及含有微量营养素和生物类黄酮、多酚和类胡萝卜素的13种添加剂(日粮11)分别饲喂8个月和10个月。测定了SAM(P8)小鼠血浆、组织匀浆和线粒体中谷胱甘肽(GSH)和谷胱甘肽二硫化物(GSSG)的含量及GSH/GSSG比值。两种饮食均有降低血浆中谷胱甘肽氧化还原作用，但饮食I对组织匀浆或线粒体中谷胱甘肽氧化还原状态的影响相对较小。值得注意的是，饮食11导致线粒体中谷胱甘肽的数量大幅增加，谷胱甘肽氧化还原状态发生明显的还原转变。总体而言，饮食11的影响是组织和性别特有的。结果表明，线粒体和组织中的谷胱甘肽氧化还原状态可以通过补充摄入相对复杂的饮食抗氧化剂混合物来改变，该混合物含有已知的诱导第二相酶、谷胱甘肽和抗氧化剂防御的物质。在与年龄相关的生理功能或寿命的有害变化中是否会出现相应的衰减尚不清楚。(C)2005 Elsevier Inc.保留所有权利。
The main purpose of this study was to investigate whether consumption of diets enriched in antioxidants attenuates the level of oxidative stress in the senescence-accelerated mouse (SAM). In separate and independent studies, two different dietary mixtures, one enriched with vitamin E, vitamin C, L-carnitine, and lipoic acid (Diet 1) and another diet including vitamins E and C and 13 additional ingredients containing micronutrients with bioflavonoids, polyphenols, and carotenoids (Diet 11), were fed for 8 and 10 months, respectively. The amounts of glutathione (GSH) and glutathione disulfides (GSSG) and GSH:GSSG ratios were determined in plasma, tissue homogenates, and mitochondria isolated from five different tissues of SAM (P8) mice. Both diets had a reductive effect in plasma; however Diet I had relatively little effect on the glutathione redox status in tissue homogenates or mitochondria. Remarkably, Diet 11 caused a large increase in the amount of glutathione and a marked reductive shift in glutathione redox state in mitochondria. Overall, the effects of Diet 11 were tissue and gender specific. Results indicated that the glutathione redox state in mitochondria and tissues can be altered by supplemental intake of a relatively complex mixture of dietary antioxidants that contains substances known to induce phase 2 enzymes, glutathione, and antioxidant defenses. Whether corresponding attenuations occur in age-associated deleterious changes in physiological functions or life span remains unknown. (c) 2005 Elsevier Inc. All rights reserved.