Functional redundancy in the yeast cell cycle: FUS3 and KSS1 have both overlapping and unique functions.
Functional redundancy in the yeast cell cycle: FUS3 and KSS1 have both overlapping and unique functions.
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酵母细胞周期中的功能冗余：FUS3 和 KSS1 具有重叠和独特的功能。
DOI：
10.1101/sqb.1991.056.01.007
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Fink,GR
中科院分区：
文献类型：
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作者：
Elion,EA;Brill,JA;Fink,GR
材料和方法培养基、质粒和菌株。如前所述制备含有2%葡萄糖、2%半乳糖或2%棉子糖的YEPD和SC培养基（参见谢尔曼等人，1986）。pYEEll 6：CEN 3 HIS 3质粒中的GAL 1-STE 4基因（Whiteway等人，1990），pYEEll 7：GAL 1-STE 12基因（来自B. Errede），pJB 207：在LEU 2 2微米载体中的Fusl-lacZ基因（Trueheart等1987），pJB 224：在LEU 2 2微米质粒中的STE 5基因。所有酵母菌株都是W303菌株EY 699 MATa FUS 3 KSS 1 ura 3 -1 his 3 -11，15 leu 2 -3，112 trpl-1 ade 2 can 1 - 100 Gal §（来自R.罗斯坦）或$288 C菌株EY 441 FUS 3 kssl ura 3 -52 1 eu 2 - 3，112 his 3 A200 ade 2 -1 lys 2 A201（Elion等，1990），除非另有说明。使用两个FUS 3无效等位基因（fus 3A
MATERIALS AND METHODSMedia, plasmids, and strains. YEPD and SC media with 2% dextrose, 2% galactose, or 2% raffinose were prepared as described previously (see Sherman et al. 1986). pYEEll6: the GAL1-STE4 gene (Whiteway et al. 1990) in a CEN3 HIS3 plasmid, pYEEll7: the GAL1-STE12 gene (from B. Errede) in a CEN3 HIS3 plasmid, pJB207: the Fusl-lacZ gene (Trueheart et al. 1987) in a LEU2 2-micron vector, pJB224: the STE5 gene in a LEU2 2-micron plasmid. All yeast strains are isogenic derivatives of W303 strain EY699 MATa FUS3 KSS1 ura3-1 his3-11, 15 leu2-3, 112 trpl-1 ade2 canl-lO0 Gal § (from R. Rothstein) or $288 C strain EY441 FUS3 kssl ura3-52 1eu2-3,112 his3A200 ade2-1 lys2A201 (Elion et al. 1990) unless noted. Two FUS3 null alleles (fus3A) were used