ONCOGENE EXPRESSION IN HUMAN HEPATOMA-CELLS PLC/PRF/5
ONCOGENE EXPRESSION IN HUMAN HEPATOMA-CELLS PLC/PRF/5
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DOI：
10.1016/0006-291x(86)90971-x
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发表时间：
1986-02-26
影响因子：
3.1
通讯作者：
BOLLON, AP
中科院分区：
文献类型：
--
作者：
MOTOO, Y;MAHMOUDI, M;BOLLON, AP
用Northern杂交方法研究了7种细胞癌基因在人肝癌细胞系PLC/PRF/5中的表达。在检测的癌基因中，c-abl、c-fes、c-fms、c-myc、c-Ha-ras和c-sis均有表达。癌基因c-ki-ras未见表达。表达的mRNAs的长度与已发表的数据几乎一致。与癌基因在Daudi淋巴瘤细胞中的表达相比，PLC/PRF/5细胞中有相同的癌基因表达，但每种癌基因表达的信号强度在Daudi细胞中强于PLC/PRF/5细胞。考虑到细胞的定位和各癌基因的功能，在肝癌细胞中的癌基因调查拓宽了对肝癌发生的认识和对人肝癌细胞特性的认识。
The expression of 7 cellular oncogenes in a human hepatoma cell line PLC/PRF/5 was studied using Northern blot analyses. Among the oncogenes tested, c-abl, c-fes, c-fms, c-myc, c-Ha-ras and c-sis were expressed. The oncogene c-Ki-ras was not expressed. The length of the mRNAs expressed was almost consistent with published data. Compared to the oncogene expression in Daudi lymphoma cells, the same kind of oncogenes were expressed in PLC/PRF/5 cells, but the intensity of the signal in each oncogene expression was stronger in Daudi cells than in PLC/PRF/5 cells. Considering the cellular localization and the function of each oncogene, the oncogene survey in hepatoma cells broadens the knowledge of hepatocarcinogenesis and the character of human hepatoma cells.