Surface projections of titanium substrates increase antithrombotic endothelial function in response to shear stress.
Surface projections of titanium substrates increase antithrombotic endothelial function in response to shear stress.
复制标题
钛基底的表面突起增加了响应剪切应力的抗血栓内皮功能。
DOI：
10.1002/jbm.a.34613
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Truskey,GeorgeA
中科院分区：
文献类型：
--
作者：
Jantzen,AlexandraE;Achneck,HardeanE;Truskey,GeorgeA
尽管机械循环辅助器械和钛（Ti）外表面镍钛合金支架具有治疗益处，但血液接触表面血栓形成的问题仍然存在。用一层内皮覆盖这些表面将模拟心血管系统的天然衬里，可能减少血栓并发症。由于已知表面形貌会影响接种细胞层的表型，并且支架和心室辅助装置会出现表面突起，因此我们检验了以下假设：与光滑钛表面相比，内皮细胞（EC）在突起高度为1.25、3和5 μm的钛表面上的功能发生了改变。在所有图案化的表面上，EC和细胞核更加对齐，并且EC更加伸长。在3和5 μm特征上，细胞面积减小。eNOS和COX 2的表达没有被图案化的表面改变，但KLF-2的表达在1.25和5 μm的特征上更高。暴露于流动后，5 μm特征上的一氧化氮产生较高。这些结果表明，在表面突起上培养的EC的一些抗血栓形成功能显著增强，并且没有功能减弱，这为将来设计用于内皮化的植入物表面提供了信息。© 2013 Wiley Periodicals，Inc. J Biomed Mater Res Part A：101A：3181-3191，2013.
Despite the therapeutic benefits of both mechanical circulatory assist devices and nitinol stents with titanium (Ti) outer surfaces, problems remain with thrombosis at the blood‐contacting surface. Covering these surfaces with a layer of endothelium would mimic the native lining of the cardiovascular system, potentially decreasing thrombotic complications. Since surface topography is known to affect the phenotype of a seeded cell layer and since stents and ventricular assist devices exhibit surface protrusions, we tested the hypothesis that endothelial cells (ECs) have altered function on Ti surfaces with protrusions of 1.25, 3, and 5 μm height, compared with smooth Ti surfaces. ECs and nuclei were more aligned and ECs were more elongated on all patterned surfaces. Cell area was reduced on the 3 and 5 μm features. Expression of eNOS and COX2 was not altered by patterned surfaces, but expression of KLF‐2 was higher on 1.25 and 5 μm features. Nitric oxide production following exposure to flow was higher on the 5 μm features. These results show that some antithrombogenic functions of ECs are significantly enhanced for ECs cultured on surface protrusions, and no functions are diminished, informing the future design of implant surfaces for endothelialization. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3181–3191, 2013.