Cloning, characterization, and heat stress-induced redistribution of a protein homologous to human hsp27 in the zebrafish Danio rerio.
Cloning, characterization, and heat stress-induced redistribution of a protein homologous to human hsp27 in the zebrafish Danio rerio.
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DOI：
10.1016/j.yexcr.2005.02.007
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发表时间：
2005-05
影响因子：
3.7
通讯作者：
L. Mao;A. Bryantsev;Maria B. Chechenova;E. Shelden
中科院分区：
文献类型：
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作者：
L. Mao;A. Bryantsev;Maria B. Chechenova;E. Shelden
热休克蛋白27是一种小分子热休克蛋白（shsp），调节应激耐受，并越来越多地被认为在组织稳态和分化中发挥作用。斑马鱼是研究发育过程的重要模型，但对这种动物中的shsps知之甚少。在这里，我们报告的序列，表达，调节和功能的斑马鱼蛋白质（zfHsp 27）同源的人Hsp 27。zfHsp 27含有三个保守的磷酸化丝氨酸和一个半胱氨酸，对细胞凋亡的调节很重要，但它缺乏大部分的C-末端尾部结构域，并在两个推定的肌动蛋白相互作用结构域中显示出低同源性，这两个结构域是哺乳动物Hsp 27的特征。zfHsp 27 mRNA在成体骨骼肌和心脏中最丰富，并且在早期胚胎发生期间上调。在哺乳动物成纤维细胞中表达的zfHsp 27在热应激和茴香霉素的反应中被磷酸化，并且这种磷酸化通过用p38 MAPK的抑制剂SB 202190处理而被阻止。zfHsp 27和人Hsp 27在哺乳动物成纤维细胞中的表达促进了相似程度的热应激耐受性。zfHsp 27融合蛋白在体外和斑马鱼胚胎中进入细胞核并与热应激细胞的细胞骨架结合。这些结果揭示了哺乳动物和硬骨鱼类Hsp 27蛋白在调控和功能上的保守性，并将斑马鱼定义为研究Hsp 27功能的新模型。
Hsp27 is a small heat shock protein (shsp) regulating stress tolerance and increasingly thought to play roles in tissue homeostasis and differentiation. The zebrafish Danio rerio is an important model for the study of developmental processes, but little is known regarding shsps in this animal. Here, we report the sequence, expression, regulation, and function of a zebrafish protein (zfHsp27) homologous to human Hsp27. zfHsp27 contains three conserved phosphorylatable serines and a cysteine important for regulation of apoptosis, but it lacks much of a C-terminal tail domain and shows low homology in two putative actin interacting domains that are features of mammalian Hsp27. zfHsp27 mRNA is most abundant in adult skeletal muscle and heart and is upregulated during early embryogenesis. zfHsp27 expressed in mammalian fibroblasts was phosphorylated in response to heat stress and anisomycin, and this phosphorylation was prevented by treatment with SB202190, an inhibitor of p38 MAPK. Expression of zfHsp27 and human Hsp27 in mammalian fibroblasts promoted a similar degree of tolerance to heat stress. zfHsp27 fusion proteins entered the nucleus and associated with the cytoskeleton of heat stressed cells in vitro and in zebrafish embryos. These results reveal conservation in regulation and function of mammalian and teleost Hsp27 proteins and define zebrafish as a new model for the study of Hsp27 function.