Comparison of microcomputed tomographic and microradiographic measurements of cortical bone porosity
Comparison of microcomputed tomographic and microradiographic measurements of cortical bone porosity
复制标题
DOI：
10.1007/s00223-003-0071-z
复制
发表时间：
2004-05-01
影响因子：
4.2
通讯作者：
Hallgrimsson, B
中科院分区：
文献类型：
--
作者：
Cooper, DML;Matyas, JR;Hallgrimsson, B
皮质骨被管道网络穿孔，这对其材料特性产生重大影响。微计算机断层扫描提供了在二维和三维上非侵入性地可视化和量化皮质孔的可能性。确定二维 (2D) 微计算机断层扫描 (muCT) 分析与分析皮质孔隙度的传统方法的比较，是更广泛采用该技术和发展三维 (3D) 分析的重要先决条件。因此，我们将二维微计算机断层扫描图像的孔隙度相关参数与匹配的显微放射线切片的参数进行了比较。以 10 微米的分辨率扫描来自 5 个人类股骨的样本，然后依次进行切片和显微射线照相。每个股骨平均生成八对图像（总共，n = 41）。针对三个参数评估了两种技术的可重复性和可比性；皮质孔隙率和平均孔隙面积 (mum(2)) 以及孔隙密度 (孔隙/mm(2))。为了可重复性。两种方法的皮质孔隙率（%）、平均孔隙面积无显着差异（P > 0.05），但孔隙密度差异显着（P < 0.001）。为了可比性，发现皮质孔隙率的方法之间的偏差（+/- 误差）为 0.51% (+/- 0.31%)，平均孔隙面积的偏差为 -155 μ m(2) (+/- 293 μ m(2))。孔隙密度的偏差取决于每平方毫米孔隙减少 14% (+/- 9.3%) 的微计算机断层扫描图像的测量尺寸。两种技术之间的定性和定量相似性证明了二维微计算机断层扫描在皮质孔隙度分析中的实用性。然而，孔密度的相对较差的结果表明，需要更高的分辨率（< 10 μm）才能一致地可视化人骨中的所有皮质孔。
Cortical bone is perforated by a network of canals that have a significant impact upon its material properties. Microcomputed tomography offers the possibility of noninvasively visualizing and quantifying cortical pores in both two and three dimensions. Establishing how two-dimensional (2D) microcomputed tomographic (muCT) analysis compares with conventional methods for analyzing cortical porosity is an important prerequisite for the wider adoption of this technique and the development of three-dimensional (3D) analysis. Therefore, we compared porosity-related parameters from 2D microcomputed tomographic images with those from matching microradiographic sections. Samples from five human femora were scanned at a 10-mum resolution and then sequentially sectioned and microradiographed. An average of eight image pairs were produced from each femur (total, n = 41). The repeatability and comparability of the two techniques was assessed for three parameters; cortical porosity and mean pore area (mum(2)), and pore density (pores/mm(2)). For repeatability. no significant difference (P > 0.05) was found between the two methods for cortical porosity (%), mean pore area, however, pore density differed significantly (P < 0.001). For comparability, the bias (+/- error) between the methods was found to be 0.51% (+/- 0.31%) for cortical porosity and -155 mu m(2) (+/- 293 mu m(2)) for mean pore area. The bias for pore density was dependent upon measurement size with microcomputed tomographic images having 14% (+/- 9.3%) fewer pores per millimeter squared. The qualitative and quantitative similarities between the two techniques demonstrated the utility of 2D microcomputed tomographic for cortical porosity analysis. However, the relatively poor results for pore density revealed that a higher resolution (< 10 mum) is needed to consistently visualize all cortical pores in human bone.