Proliferation and osteogenic differentiation of rat bone marrow stromal cells on bioapatite with different crystalline facets
Proliferation and osteogenic differentiation of rat bone marrow stromal cells on bioapatite with different crystalline facets
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DOI：
10.1002/jbm.a.32569
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发表时间：
2010-05-01
影响因子：
4.9
通讯作者：
Tabata, Yasuhiko
中科院分区：
文献类型：
--
作者：
Ichinohe, Norihisa;Nakano, Takayoshi;Tabata, Yasuhiko
本研究的目的是研究大鼠骨髓基质细胞（MSC）和MC 3 T3-E1细胞在不同晶面的生物磷灰石（Ap）上的附着、成骨分化和胶原基质分泌。通过分别平行和垂直于纵向切割热处理的牛股骨皮质骨，制备具有a-小关节面和c-小关节面表面的Ap样品。MC 3 T3-E1细胞在a-Ap样品上铺展贴附，而在c-Ap上细胞形状为圆形。在两个样品之间没有观察到MSC和MC 3 T3-E1细胞的增殖特征的差异。a-Ap样品上的两种细胞的碱性磷酸酶活性均显著高于c-Ap样品。在a-Ap样品上，从MC 3 T3-E1细胞分泌的胶原基质与c-Ap的胶原基质相反，沿着Ap的c轴取向。细胞倾向于沿着胶原蛋白方向排列。可以得出结论，Ap样品的胶原纤维取向影响细胞的排列。(C)2009 Wiley Periodicals，Inc. J Biomed Mater Res 93A：646-655，2010
The objective of this study is to investigate the attachment, osteogenic differentiation, and collagen matrix secretion of rat bone marrow stromal cells (MSC) and MC3T3-E1 cells on the biological apatite (Ap) with different crystalline facets. The Ap samples with a-facet and c-facet surfaces were prepared by cutting the heat-treated cortical bone of bovine femurs parallel and perpendicular to the longitudinal direction, respectively. MC3T3-E1 cells attached spreading on the a-Ap sample whereas their shape of cells was round on the c-Ap. No difference in the proliferation profile of MSC and MC3T3-E1 cells was observed between the two samples. The activity of alkaline phosphatase was significantly higher for both the cells on the a-Ap sample than that of c-Ap one. The matrix of collagen secreted from MC3T3-E1 cells on the a-Ap sample oriented along the c-axis of Ap, in contrast to those of c-Ap. The cells tended to align along the collagen orientation. It is concluded that the alignment of cells is influenced by the collagen fibril orientation of Ap samples. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 646-655, 2010