Analysis of 76 kb of the chlorella virus PBCV-1 330-kb genome: map positions 182 to 258.
Analysis of 76 kb of the chlorella virus PBCV-1 330-kb genome: map positions 182 to 258.
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小球藻病毒 PBCV-1 330-kb 基因组的 76 kb 分析：图谱位置 182 至 258。
DOI：
10.1006/viro.1996.0482
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
VanEtten,JL
中科院分区：
文献类型：
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作者：
Kutish,GF;Li,Y;Lu,Z;Furuta,M;Rock,DL;VanEtten,JL
对330kb小球藻病毒PBCV-1基因组中位于182 - 258 kb位点之间的76kb新测序DNA进行分析，发现175个开放阅读框（orf），包含65个或更长密码子。这175个orf中有105个被认为是主要orf。数据库中105个主要orf中有21个相似蛋白，包括核糖核苷酸还原酶小亚基、RNase III、硫氧还蛋白、glutaredoxin、蛋白二硫异构酶、脱氧核苷激酶、青蛙病毒3 atp酶、乙酰细菌纤维素合成酶、噬菌体编码的核酸内切酶和两个C-5胞嘧啶DNA甲基转移酶。其中一个orf是PBCV-1主要衣壳蛋白。105个主要orf沿基因组均匀分布。一组orf被543个核苷酸分开，而75组orf被少于100个核苷酸分开。175个orf中有19个与其他PBCV-1 orf相似，表明它们代表基因重复或基因家族。
Analysis of 76 kb of newly sequenced DNA, located between map positions 182 and 258 kb in the 330-kb chlorella virus PBCV-1 genome, revealed 175 open reading frames (ORFs) of 65 codons or longer. One hundred and five of these 175 ORFs were considered major ORFs. Twenty-one of the 105 major ORFs resembled proteins in databases including ribonucleotide reductase small subunit, RNase III, thioredoxin, glutaredoxin, protein disulfide isomerase, deoxynucleoside kinase, frog virus 3 ATPase,Acetobactercellulose synthase, a bacteriophage encoded endonuclease, and two C-5 cytosine DNA methyltransferases. One of the ORFs was the PBCV-1 major capsid protein. The 105 major ORFs were evenly distributed along the genome. One set of ORFs was separated by 543 nucleotides whereas 75 of the ORFs were separated by fewer than 100 nucleotides. Nineteen of the 175 ORFs resembled other PBCV-1 ORFs, suggesting that they represent either gene duplications or gene families.