Cell lineage tracing during Xenopus tail regeneration
Cell lineage tracing during Xenopus tail regeneration
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DOI：
10.1242/dev.01155
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发表时间：
2004-06-01
期刊：
影响因子：
4.6
通讯作者：
Slack, JMW
中科院分区：
文献类型：
--
作者：
Gargioli, C;Slack, JMW
非洲爪蟾蝌蚪的尾巴将再生后截肢，和所有三个主要的轴结构-脊髓，脊索和分段肌节中发现再生的尾巴。我们已经研究了这三种组织类型在再生过程中的细胞起源。我们产生了CMV（猿猴巨细胞病毒）启动子驱动GFP（绿色荧光蛋白）在整个胚胎中无处不在的非洲爪蟾胚胎转基因。然后通过在神经胚阶段从转基因供体到未标记的宿主进行移植来特异性标记单个组织。当宿主发育成蝌蚪时，它们携带一个用GFP标记的适当组织区域。通过标记区域切断这些尾部，并跟踪标记细胞在再生体中的分布。我们还用Cre-lox方法标记了肌纤维，结果表明脊髓和脊索是从断肢中同一组织类型再生的，没有标记其他组织。在肌肉的情况下，我们表明，再生的肌纤维来自卫星细胞，而不是来自预先存在的肌纤维。这表明分化的细胞类型之间的化生不会发生，并且非洲爪蟾尾部再生的过程更类似于哺乳动物中的组织更新而不是有尾目动物尾部再生。
The tail of the Xenopus tadpole will regenerate following amputation, and all three of the main axial structures - the spinal cord, the notochord and the segmented myotomes are found in the regenerated tail. We have investigated the cellular origin of each of these three tissue types during regeneration.We produced Xenopus laevis embryos transgenic for the CMV (Simian Cytomegalovirus) promoter driving GFP (Green Fluorescent Protein) ubiquitously throughout the embryo. Single tissues were then specifically labelled by making grafts at the neurula stage from transgenic donors to unlabelled hosts. When the hosts have developed to tadpoles, they carry a region of the appropriate tissue labelled with GFP. These tails were amputated through the labelled region and the distribution of labelled cells in the regenerate was followed. We also labelled myofibres using the Cre-lox method.The results show that the spinal cord and the notochord regenerate from the same tissue type in the stump, with no labelling of other tissues. In the case of the muscle, we show that the myofibres of the regenerate arise from satellite cells and not from the pre-existing myofibres. This shows that metaplasia between differentiated cell types does not occur, and that the process of Xenopus tail regeneration is more akin to tissue renewal in mammals than to urodele tail regeneration.