Stable RK2-derived cloning vectors for the analysis of gene expression and gene function in gram-negative bacteria
Stable RK2-derived cloning vectors for the analysis of gene expression and gene function in gram-negative bacteria
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DOI：
10.1094/mpmi.2001.14.3.426
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发表时间：
2001-03-01
影响因子：
3.5
通讯作者：
Michiels, J
中科院分区：
文献类型：
--
作者：
Dombrecht, B;Vanderleyden, J;Michiels, J
本文报道了几种稳定的rk2克隆载体的构建，用于分析革兰氏阴性菌的基因表达和功能。质粒稳定性由RK2位点或从根瘤菌sp. NGR234的pNGR234a插入的spsAB或spsCD共生质粒稳定性位点赋予。载体携带多个克隆位点，对载体序列的转录活性具有保护作用。含有组成型nptII启动子或无启动子gusA基因的载体衍生物适合于研究细菌中基因的功能或调控。
The construction of several stable RK2-derived cloning vectors for the analysis of gene expression and function in gram-negative bacteria is reported. Plasmid stability is Conferred by the RK2 par locus or by insertion of the spsAB or spsCD symbiotic plasmid stability loci from pNGR234a of Rhizobium sp. NGR234. The vectors carry multiple cloning sites with protection against read-through transcriptional activity of vector sequences. Vector derivatives with the constitutive nptII promoter or a promoter-less gusA gene are suitable for the study of gene function or regulation in bacteria.