Functionally rodless mice: transgenic models for the investigation of cone function in retinal disease and therapy
Functionally rodless mice: transgenic models for the investigation of cone function in retinal disease and therapy
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DOI：
10.1016/s0042-6989(01)00214-0
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发表时间：
2002-02-01
期刊：
影响因子：
1.8
通讯作者：
Pugh, EN
中科院分区：
文献类型：
--
作者：
Lyubarsky, AL;Lem, J;Pugh, EN
利用两个基因工程小鼠品系，在没有视杆驱动反应干扰的情况下，对小鼠视锥细胞的视网膜电活动进行了研究：(1)视杆转导蛋白α亚基基因缺失的小鼠(转导蛋白α-/-)和(2)视杆转导蛋白缺失的小鼠(arrestin-/-)。在出生后的头三个月里，两个菌株都有正常的杆状结构和正常的杆状结构，但转导蛋白α/-小鼠对光没有杆状驱动的反应，而arrestin/-小鼠的杆状驱动的活动可以被一个强烈的闪光抑制数小时。作为对强烈闪光的反应，这些品系小鼠的视网膜电信号显示出一个容易识别的纯锥a波，其幅度类似于10-V饱和幅度。一个530 nm的背景饱和了野生型小鼠的杆状反应，发现这两个转基因系小鼠的b波反应是不敏感的。B波是由M-或UV-锥体颜料捕获的光子驱动的。530 nm背景对紫外光驱动的反应的减敏作用为主要表达紫外光的视锥细胞中M-色素的功能共表达假说提供了新的证据。(C)2002年由爱思唯尔科学有限公司出版。
Two genetically engineered strains of mice were used to characterize murine cone function electroretinographically, without interference of rod-driven responses: ( 1) mice with a deletion of the gene for the rod transducin alpha-subunit (transducin alpha-/-) and (2) mice with rod arrestin deleted (arrestin -/-). In the first three months of age, both strains have a normal complement of rods and normal rod structure, but transducin alpha-/- mice have no rod-driven responses to light, while rod-driven activity of arrestin -/- mice can be suppressed by a single intense flash for hours. In response to intense flashes the electroretinograms of these strains of mice showed a readily identifiable, pure-cone a-wave of similar to10 muV saturating amplitude. A 530 nm background that saturates rod responses of wild type mice was found to desensitize the b-wave responses of mice of both transgenic lines. whether the b-waves were driven by photons captured by M- or UV-cone pigments. The desensitizing effect of the 530 nm background on UV-pigment driven responses provides new evidence in support of the hypothesis of functional co-expression of the M-pigment in cones expressing primarily the UV-pigment. (C) 2002 Published by Elsevier Science Ltd.