Formation of S-[2-(N7-guanyl)ethyl] adducts by the postulated S-(2-chloroethyl)cysteinyl and S-(2-chloroethyl)glutathionyl conjugates of 1,2-dichloroethane.
Formation of S-[2-(N7-guanyl)ethyl] adducts by the postulated S-(2-chloroethyl)cysteinyl and S-(2-chloroethyl)glutathionyl conjugates of 1,2-dichloroethane.
复制标题
通过假定的 1,2-二氯乙烷的 S-(2-氯乙基)半胱氨酰和 S-(2-氯乙基)谷胱甘肽缀合物形成 S-[2-(N7-鸟苷基)乙基]加合物。
DOI：
10.1021/bi00381a035
复制
发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Reed,DJ
中科院分区：
文献类型：
--
作者：
Foureman,GL;Reed,DJ
材料和方法所用的所有化学品均为可从商业来源获得的最高等级。S-（2-氯乙基）谷胱甘肽（CEG）和S-（2-氯乙基）-L-半胱氨酸（CEC）的合成如Reed和Foureman（1986）以及Schasteen和Reed（1983）中所报道。如通过高效液相色谱法（HPLC）测定的，CEG为90%纯，主要污染物为水解产物;根据相同标准，CEC为> 95%纯。通过将227 µ CEC（42 mg）或CEG（85 mg）作为干粉加入4 mL中，完成用于化学分析的脱氧鸟苷加合物的大量制备
Materials and MethodsAll chemicals used were of the highest grade available from commercial sources. The synthesis of both S-(2-chloroethyl) glutathione (CEG) and S-(2-chloroethyl)-L-cysteine (CEC) was as reported in Reed and Foureman (1986) and in Schasteen and Reed (1983). The CEG was 90% pure as determined by high-performance liquid chromatography (HPLC), the main contaminant being the hydrolysis product; CEC was> 95% pure by the same criteria. Bulk preparation of the deoxyguanosine adducts for chem-ical analysis was accomplished by adding, as the dry powder, 227 µ of either CEC (42 mg) or CEG (85 mg) to 4 mL