The position- and lymphatic lumen-controlled tissue chambers to study live lymphatic vessels and surrounding tissues ex vivo.
The position- and lymphatic lumen-controlled tissue chambers to study live lymphatic vessels and surrounding tissues ex vivo.
复制标题
位置和淋巴管腔控制的组织室，用于离体研究活体淋巴管和周围组织。
DOI：
10.1089/lrb.2014.0020
复制
发表时间：
2014
影响因子：
1.4
通讯作者：
Gashev,AnatoliyA
中科院分区：
文献类型：
--
作者：
Maejima,Daisuke;Nagai,Takashi;Bridenbaugh,EricA;Cromer,WalterE;Gashev,AnatoliyA
背景：到目前为止，还没有可用的工具为淋巴研究人员提供在组织位置和淋巴管腔控制的条件下在含有淋巴管的组织外植体中进行实验的能力。方法和结果：在本文中，我们提供了使用新开发和实施的位置和淋巴管腔控制的组织室来研究活体淋巴管及其周围的方法的技术细节和描述。 组织离体。在这项研究中，我们首次对位于安装在新组织室中的组织外植体中的大鼠肠系膜淋巴管（MLV）和维持在隔离血管设置中的分离、插管和加压的大鼠MLV的收缩和泵送活性进行了详细的比较分析。我们发现组织室中的 MLV 和孤立的 MLV 的跨壁压力和壁剪切应力敏感性的影响没有显着差异。结论：我们得出的结论是，这种新的实验工具（位置和淋巴管腔控制的组织室）可以精确研究 MLV 与组织微环境元素相互作用的淋巴功能。该方法提供了一套重要的新实验工具来研究淋巴功能。
Background:Until now, there has been no tool available to provide lymphatic researchers the ability to perform experiments in tissue explants containing lymphatic vessels under tissue position- and lymphatic lumen-controlled conditions.Methods and Results:In this article we provide technical details and description of the method of using the newly developed and implemented the position- and lymphatic lumen-controlled tissue chambers to study live lymphatic vessels and surrounding tissuesex vivo. In this study, we, for the first time, performed detailed comparative analysis of the contractile and pumping activity of rat mesenteric lymphatic vessels (MLVs) situated within tissue explants mounted in new tissue chambers and isolated, cannulated, and pressurized rat MLVs maintained in isolated vessel setups. We found no significant differences of the effects of both transmural pressure- and wall shear stress sensitivities of MLVs in tissue chambers and isolated MLVs.Conclusions:We conclude that this new experimental tool, a position- and lymphatic lumen-controlled tissue chamber, allows precise investigation of lymphatic function of MLVs interacting with elements of the tissue microenvironment. This method provides an important new set of experimental tools to investigate lymphatic function.